Calcium Flux & FLIPR Assay Service for GPCR-Ligand Interacting Detection
Introduction Strategies What We Can Offer Workflow Required Materials Highlights Customer Reviews FAQs Extended Services
Accelerate Your Drug Discovery Process!
G-protein coupled receptors (GPCRs) are integral membrane proteins that play a critical role in cellular communication and signaling. Calcium flux assays using FLIPR (Fluorescence Imaging Plate Reader) technology offer a sensitive and versatile method for monitoring GPCR activation by ligands. These assays have been widely adopted in drug discovery to screen for potential therapeutic agents targeting GPCRs. Recent advancements in FLIPR technology have made it an indispensable tool for high-throughput screening in both academia and industry.
Are you currently facing challenges in high-throughput screening, GPCR-related drug discovery, or difficulty in detecting ligand interactions in complex cellular environments? Our calcium Flux and FLIPR assay service provides a robust solution to identify novel GPCR-targeted drugs with high specificity and efficacy through our optimized high-throughput screening platforms. By utilizing state-of-the-art FLIPR technology, we ensure that you can detect even the most subtle ligand-induced changes in calcium flux. This capability is crucial for drug discovery programs targeting diseases related to GPCRs, including cancer, cardiovascular diseases, and neurological disorders.
Strategies
To effectively measure the interaction between GPCRs and ligands, the use of calcium flux assays in combination with FLIPR screening provides several key advantages:
High Sensitivity
The ability to detect even subtle changes in intracellular calcium levels enables precise monitoring of GPCR activation, providing a highly sensitive measure of ligand-receptor interactions. This sensitivity is crucial for detecting weak or transient ligand binding, ensuring that even low-affinity interactions can be captured.
High-throughput Screening
The FLIPR systems support the analysis of hundreds to thousands of samples simultaneously, offering unparalleled efficiency in screening large compound libraries. This capability significantly reduces the time and cost associated with traditional assays, making it ideal for early-stage drug discovery and optimizing lead compounds rapidly.
Real-time Data
With real-time monitoring of ligand-GPCR interactions, the assay allows researchers to capture dynamic biological events as they occur. This real-time capability provides insights into the kinetics and pharmacodynamics of ligand-receptor binding, allowing for the detailed characterization of compounds, including their potency, efficacy, and response time.
Versatility
FLIPR calcium flux assays are applicable to a wide range of GPCR targets. This versatility makes the assay platform suitable for a variety of therapeutic areas, such as oncology, neurodegenerative diseases, cardiovascular research, and immunology. Whether you're screening for agonists, antagonists, or allosteric modulators, our platform adapts to the specific needs of your drug discovery project.
Quantitative and Reliable Results
The combination of high-throughput screening and calcium flux measurement provides accurate and reproducible data that supports quantitative analysis of ligand interactions. This is crucial for identifying potential drug candidates and optimizing them for further preclinical development.
Assay Customization and Optimization
Our platform supports the customization of assay protocols for specific experimental needs, such as adjusting the calcium indicator, modifying screening conditions, and fine-tuning the readout to suit different GPCR types. This flexibility ensures that the assays meet the requirements of your specific research, allowing for optimized and tailored results.
What We Can Offer
At Creative Biolabs, we provide comprehensive calcium Flux and FLIPR assay services that support your research and drug development projects. Our services include:
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Custom Assay Development: Tailored assays specific to your GPCR targets, optimized for the detection of ligand binding and receptor activation.
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High-Throughput Screening: Using advanced FLIPR technology, we enable efficient screening of compound libraries to identify lead candidates with high specificity and potency.
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Expert Data Analysis: Our team of specialists provides in-depth analysis of screening results, including hit identification and signal pathway interpretation.
Workflow
1. Assay Development
Our experts design and optimize the calcium flux assay specific to your GPCR target, ensuring accurate detection of ligand-receptor interactions.
2. Compound Screening
Compounds are screened using the FLIPR system, where calcium flux is monitored in real-time to identify potential ligands.
3. Data Collection & Analysis
Data is collected using high-throughput technologies, followed by expert analysis to identify hits, analyze dose-response relationships, and evaluate compound efficacy.
4. Hit Confirmation
Hits identified in screening are subjected to secondary assays to confirm their activity, ensuring reliability in further studies.
5. Report Generation
A detailed report is provided, summarizing the findings, including compound profiles and suggested next steps for further research.
Required Starting Materials
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GPCR Expression Constructs: These constructs are crucial for expressing the target GPCR in host cells to study ligand-GPCR interactions. If not already available, they need to be synthesized or provided for the assay.
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Compound Library: A collection of diverse compounds (small molecules, peptides, or biologics) to screen for potential ligands of the target GPCR. The library can be tailored to specific goals or be a broad, high-throughput set of compounds.
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Cell Line Information: The host cell line should be appropriate for expressing the GPCR of interest. If a suitable cell line isn't available, one will need to be engineered or selected to ensure effective expression and functionality.
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Calcium Indicator: A calcium-sensitive dye (e.g., Fluo-4) is needed to measure intracellular calcium changes. If not already incorporated into the cell line, it must be introduced for accurate readout.
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Reagents and Buffer Solutions: Various reagents and buffers are required, including those for cell maintenance, calcium buffering, and assay conditions. These ensure optimal conditions for cell viability and receptor activity during screening.
Highlights
Comprehensive Support
Our team of experts works closely with you to design assays, optimize conditions, and deliver insightful analysis that accelerates your research and development process.
Customizable Solutions
Whether you're working with a specific GPCR target or need to screen a large library of compounds, our services can be fully customized to suit your project's unique requirements. We offer flexible experimental setups that cater to various targets, assays, and high-throughput screening needs, ensuring the best fit for your research objectives.
Advanced Technology
Leveraging the latest in FLIPR and high-throughput technologies, we ensure fast, accurate, and reproducible results. Our state-of-the-art instrumentation and techniques guarantee reliable data for the most demanding screening projects, helping you identify key hits and optimize lead compounds efficiently.
Expert Consultation and Troubleshooting
With years of experience in assay optimization, hit identification, and compound screening, we provide expert consultation and troubleshooting to overcome any challenges that arise during the process. This expertise ensures you get the most out of your assays, reducing the time and cost involved in compound screening.
Experience the Creative Biolabs Advantage - Get a Quote Today.
Customer Reviews
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Enhanced GPCR Screening: "Using Creative Biolabs' Calcium Flux and FLIPR Assay has significantly improved our ability to screen for novel GPCR ligands. The high-throughput capabilities allowed us to efficiently analyze hundreds of compounds in a short time, accelerating our lead identification process." – 2025, Dr. J. M
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Accurate Calcium Flux Detection: "The tailored calcium flux assay for our GPCR target provided highly reproducible data. It helped us validate key compounds with promising pharmacological profiles. The service was crucial in advancing our drug development project." – 2025, Dr. T. L
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Efficient and Reliable Service: "Creative Biolabs' FLIPR screening service enabled us to identify specific agonists for a previously challenging GPCR target. Their proficiency in data analysis and assay development proved to be extremely valuable. – 2025, Dr. R. K
FAQs
Q: What is the primary benefit of using FLIPR assays for GPCR research?
A: FLIPR assays provide high sensitivity and high throughput, allowing for the detection of real-time changes in intracellular calcium flux, making them ideal for studying GPCR activation and ligand interactions.
Q: What types of GPCRs can be studied using the Calcium Flux and FLIPR Assay?
A: Our services are compatible with a wide range of GPCRs, including those involved in neurological, cardiovascular, and cancer-related pathways.
Q: How long does it take to develop a custom GPCR calcium flux assay?
A: Custom assay development typically takes 3-4 weeks, depending on the complexity of the target and the cell systems used.
Q: Can I screen my own compound library?
A: Yes, we can screen your compound library using our high-throughput FLIPR system to identify potential lead candidates for your target GPCRs.
Q: What is the level of data analysis provided with the service?
A: We provide comprehensive data analysis, including dose-response curves, hit identification, and pathway interpretation, ensuring you gain actionable insights from your screening results.
Extended Services
In addition to our calcium Flux and FLIPR assay service, Creative Biolabs offers several complementary services to enhance your drug discovery process:
Recombinant Protein Expression
Our integrated platform enables robust, high-yield recombinant expression of complex targets such as GPCRs, ensuring structural integrity and functional activity for downstream biochemical and pharmacological analysis.
Learn More →
Functional Assays
Through a comprehensive suite of customized functional bioassays, we assess ligand-induced signaling cascades, receptor activity, and immune cell responses, providing detailed mechanistic insights to accelerate therapeutic discovery and validation.
Learn More →
If you're ready to accelerate your GPCR drug discovery, contact our team today to discuss how our calcium Flux and FLIPR assay service can help you achieve your project goals.
For Research Use Only | Not For Clinical Use
