Overexpression Cell Line Products
An overexpression cell line is a genetically modified cell line designed to express a specific gene at significantly higher levels than normal. This is achieved by introducing a gene of interest into a suitable expression vector, typically containing a strong promoter for enhanced transcription. Overexpression cell lines are widely used in molecular biology and biotechnology to study the functions and characteristics of proteins, investigate gene regulatory mechanisms, and explore the consequences of increased gene activity. Researchers often utilize these cell lines to analyze cellular processes such as proliferation, differentiation, and response to drugs, making them valuable tools for drug discovery, disease modeling, and therapeutic development. By providing insights into the molecular underpinnings of various biological phenomena, overexpression cell lines play a crucial role in advancing our understanding of genetics and cellular biology.
Advantages of Overexpression Cell Lines
Enhanced Protein Production
Facilitating biochemical studies, structural biology research, and protein purification for various applications.
Functional Studies
Providing insights into signaling pathways, metabolic functions, and other essential cellular mechanisms.
Disease Modeling
Helping to elucidate disease mechanisms and identify potential therapeutic targets.
Pathway Analysis
Allowing for the identification of upstream regulators and downstream effects.
Drug Response Assessment
Evaluate how increased expression of target proteins affects cellular responses to drugs, including sensitivity or resistance, which is crucial in drug discovery and development.
Genetic Screening
Combined with high-throughput screening techniques to find substances that alter the overexpressed protein's activity, assisting in the development of novel medicinal treatments.
Featured Products
| Cat. NO | Product Name | Cell Line | Overexpressed Gene |
|---|---|---|---|
| ITS-0624-HMM1 | CellOnco™ HEK293T-Tg(Human A1 Receptor) Stable Cell Line | HEK293T | A1 Receptor |
| ITS-0624-HMM73 | CellOnco™ HEK293T-Tg(Human CB1 Receptor) Stable Cell Line | HEK293T | CB1 Receptor |
| ITS-0822-HMM34 | PharmaOnco™ CHO-K1/CD112 Stable Cell Line | CHO-K1 | CD112 |
| ITS-0822-HMM37 | PharmaOnco™ CHO-K1/Mouse CD155 Stable Cell Line | CHO-K1 | CD155 |
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Applications of Overexpression Cell Lines
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Molecular Biology
Studying gene function and regulation, including the effects of gene overexpression on cellular pathways and mechanisms. -
Cancer Research
Examining how tumor suppressor genes and oncogenes affect the development, spread, and response to treatment of cancer. -
Stem Cell Biology
Exploring the effects of overexpressing transcription factors on stem cell pluripotency, self-renewal, and differentiation. -
Drug Discovery and Development
Validating potential therapeutic targets by assessing the effects of drug candidates on cell lines with overexpressed target proteins. -
Neuroscience
Studying the effects of gene overexpression on neuronal function, synaptic plasticity, and neurodegenerative diseases.
Workflow of Overexpression Cell Lines
Published Data
Fig.1 The figure above shows the generation protocol for an overexpressed cell line.1
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Reference
- Jeffery, Daniel et al. "CENP-A overexpression promotes distinct fates in human cells, depending on p53 status." Communications biology vol. 4,1 417. Distributed under Open Access license CC BY 4.0, without modification.