As one of the current most popular immunotherapies, CAR-T therapy shows its strength by boosting the patients’ own immune system to destroy the cancer. Creative Biolabs provides a comprehensive series of services in cellular and gene therapies including T Cell Receptor (TCR) T cell therapy, CAR-T cell therapy, CAR-macrophage cell therapy, Chimeric Antigen Receptor (CAR) NK Cell Therapy supported by our multidisciplinary team with flexible solutions. We can accommodate the specific properties of your product and provide flexible integrated solutions.
Fig.1 Treatment of patients with B-cell malignancies using anti-CD19 CAR T cells. (Klebanoff, 2014)
“We ensure careful consideration for the development of CAR-T therapy product to achieve efficient execution and regulatory approval.”
Chimeric antigen receptors (CARs) are recombinant receptor constructs which are composed of an extracellular single-chain variable fragment (scFv) derived from an identified antibody specific for tumor neoantigen, joined to a hinge or spacer peptide and a transmembrane domain, which is further linked to the intracellular T cell signaling domains of the T cell receptor. Combining the specificity of an antibody with the cytotoxic and memory functions of T cells, CAR-T cell therapy shows powerful and promising therapeutic functions for cancer treatment in high affinity without MHC dependent requirement (see Fig.1).
Fig.2 Structural components of CAR (left) and gene modified CAR-T cell therapy(right). (Harris, 2016; Garber, 2018)
“We are dedicated to accelerating your TCR-T therapy product through a series of innovative and advanced platforms.”
CAR-T cell therapy has been considered the most significant therapeutic advance in a generation. To develop CAR-T based approaches, Creative Biolabs offers an end-to-end integrated solution covering all steps prior to clinical trials (see Fig.2). You can start at any module and request a flexible integration of different modules to fit your projects. Both engineering services for αβ T cell and γδ T cell derived from patients and normal populations can be achieved.
Fig.3 Process of CAR-engineered T cell therapy.
With multiple gene and protein analysis technologies, we offer unique de novo neoantigen discovery, identification, selection to get a suitable CAR-T therapy target with high potency and low toxicity.
Aided by our in-house antibody database and various types of libraries including hybridomas, mouse, rabbit and human, plus scFv sequencing and affinity measurement platforms, we are dedicated to generating the most optimized scFv with high affinity and good properties.
We are capable of designing and constructing different generations of CARs, also various types of engineered CARs are available at Creative Biolabs, such as suicide CAR, inducible CAR, enhanced CAR, bispecific CAR, synNotch CAR, masked CAR, oxygen-sensing CAR, etc.
With various transfection technologies including electroporation, as well as cell transduction though adenovirus, lentivirus retroviral, etc., we offer CAR gene packaging and delivery services for CAR-T cell generation. To facilitate your drug development, both GLP and GMP level can be achieved to fit your purpose of the project.
For generated CAR-T products, a wide range of in vitro assays are available to evaluate the efficiency of CAR-T product, including CAR expression validation, CAR-T cell proliferation, cytokine release assay, cytotoxicity assay, etc.
Once in vitro assays are completed, a series of in vivo testing systems will be performed to evaluate the CAR-T product, including animal models, biodistribution studies, efficacy testing, toxicology assessment and other GLP compliant assays.
Fig.4 End-to-end CAR-T Integrated Solutions.
“Feel free to speak with our CAR-T cell therapy experts everywhere, everyday.”
With the commitment of being your best CAR-T therapy partner, Creative Biolabs has established the utmost efficient integrated solutions to innovate and accelerate your drug developments for various types of cancers especially the hematologic malignancies. Please feel free to contact us to get more information and a quote for any CAR-T therapy module.
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