CytoFinal™ APAF-1-ALT Cell ELISA Kit

CAT#: ITS-0326-WB81
Product Type: ELISA Kit
Species: Human
Target: Apoptotic protease-activating factor 1 (APAF-1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects APAF-1 activation in cultured cells to assess apoptosis signaling dynamics.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ APAF-1-ALT Cell ELISA Kit provides a highly sensitive, cell-based method to measure apoptotic protease-activating factor 1 (APAF-1) activity directly in cultured cells. By combining immunodetection with an enzymatic ALT readout, the kit enables accurate evaluation of intrinsic apoptosis, facilitating studies of cell death mechanisms, drug responses, and cytotoxicity.
Features
High specificity: Selectively detects APAF-1 activation in intact cells without interference from other apoptotic or metabolic proteins.
Sensitive detection: Detects subtle changes in apoptosis induction, suitable for low-expression cells or early time-point experiments.
Cell-based format: Directly measures APAF-1 activity in cultured cells, eliminating the need for cell lysis and preserving physiological context.
Complete kit: Includes all reagents, optimized buffers, and a microplate-compatible protocol for efficient, reproducible workflow.
Process Relevance
Monitors APAF-1 activation to evaluate intrinsic apoptosis and cytotoxic responses in drug development and cellular research.
Application Stage
Preclinical research, mechanistic studies of apoptosis, cytotoxicity screening, and pathway validation.
Applications
Quantifies APAF-1 activity in cultured cells for apoptosis evaluation, drug efficacy testing, cytotoxicity assessment, ADME profiling studies, and mechanistic studies of intrinsic cell death.
Target
Apoptotic protease-activating factor 1 (APAF-1)
Detection Method
Colorimetric
Sample Type
Cell lysates, tissue homogenates, and other biological fluids.
Assay Measures
Quantitative
Research Areas
Apoptosis research, oncology therapeutics, neurodegeneration studies, pharmacokinetics (ADME), immunology, drug discovery, cell signaling pathway analysis, etc.
Size
96 tests
Data Output Type
Quantitative measurement of APAF-1 enzymatic activation using colorimetric ALT signal, endpoint or kinetic analysis.
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
APAF-1 is a central mediator of intrinsic apoptosis, forming the apoptosome and activating caspase-9. Its regulation is critical for understanding cell death, disease pathogenesis, and drug responses in vitro.
Pathways
APAF-1 functions within the intrinsic apoptosis pathway, linking mitochondrial cytochrome c release to caspase-9 activation, ultimately driving downstream executioner caspases and programmed cell death.
For Research Use Only | Not For Clinical Use
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