CytoFinal™ Low Sample Volume Rat Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB93
Product Type: ELISA Kit
Species: Rat
Target: Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies cytoplasmic AST (GOT1) in rat samples using minimal volumes for efficient preclinical assessment.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Rat AST (GOT1) ELISA Kit enables sensitive detection of cytoplasmic AST from limited rat serum, plasma, tissues, or cell lysates. Designed for low-volume samples, it facilitates high-precision metabolic and hepatocellular assessments in preclinical studies.
Features
Requires minimal sample input while maintaining high sensitivity, enabling accurate AST quantification in limited-volume rat samples.
High specificity with sandwich ELISA technology ensures selective detection of cytoplasmic AST without cross-reactivity from mitochondrial isoforms.
Reagents are standardized and quality-controlled, providing consistent reproducibility across diverse rat sample types and experimental designs.
Rapid colorimetric readout allows efficient processing, supporting multiple samples simultaneously and reliable low-level AST detection.
Process Relevance
Enables accurate measurement of cytoplasmic AST in limited samples, improving hepatocellular function monitoring in preclinical studies.
Application Stage
Applicable in preclinical research, drug safety testing, hepatology studies, and small-sample laboratory investigations.
Applications
Sensitive detection of cytoplasmic AST in limited rat serum, plasma, tissues, or cell lysates for hepatotoxicity evaluation and ADME profiling studies.
Target
Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, preclinical pharmacology, metabolism, enzymology, biomarker discovery, pharmacokinetics (ADME), toxicology, translational research, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST levels in minimal-volume rat samples.
Assay Range
7.8 ng/ml - 500 ng/ml
LOD
< 2.9 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 catalyzes reversible transamination between aspartate and α-ketoglutarate, linking amino acid metabolism to TCA cycle activity. Cytoplasmic AST levels indicate hepatocellular integrity and metabolic status, critical for preclinical assessment and toxicology studies.
Pathways
AST participates in the malate-aspartate shuttle, regulating cytoplasmic-mitochondrial redox balance. It contributes to TCA cycle flux, amino acid homeostasis, and energy production in hepatocytes and other metabolically active cells.
For Research Use Only | Not For Clinical Use
Online Inquiry