CytoFinal™ Cow Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB101
Product Type: ELISA Kit
Species: Cow
Target: Mitochondrial Aspartate Aminotransferase (GOT2)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures mitochondrial AST (GOT2) in cow tissues, cell lysates, and biological fluids.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Cow AST (GOT2) ELISA Kit offers a sensitive, colorimetric assay for in vitro quantification of mitochondrial AST in cow-derived tissue homogenates, cell lysates, and other biological fluids. The kit provides a reliable, reproducible platform for monitoring GOT2 activity under various experimental conditions.
Features
High sensitivity detection with a dynamic range of 0.156-10 ng/mL, enabling precise quantification of mitochondrial AST levels.
Sandwich ELISA format ensures excellent specificity using pre-coated capture antibodies and biotin-HRP detection reagents.
Lyophilized standards and stable reagents maintain consistent performance with minimal activity loss under proper storage conditions.
Optimized for multiple sample types including tissue homogenates, cell lysates, and various biological fluids for versatile applications.
Process Relevance
Monitors mitochondrial AST to assess cellular metabolic function and tissue-specific enzymatic activity in experimental models.
Application Stage
Drug discovery, metabolic pathway analysis, and in vitro experimental validation.
Applications
Applicable for quantitative ELISA assays using cow tissue homogenates, cultured cell lysates, and biological fluids, supporting metabolic, toxicological, and ADME profiling studies.
Target
Mitochondrial Aspartate Aminotransferase (GOT2)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolism, pharmacokinetics (ADME), mitochondrial function, enzymology, veterinary biochemistry, toxicology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of enzyme concentration via optical density at 450 nm.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Cow
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Mitochondrial AST (GOT2) catalyzes the reversible transamination between aspartate and α-ketoglutarate, linking amino acid metabolism to the TCA cycle. Its activity reflects cellular energy states and mitochondrial health, making it a critical biomarker in metabolic research.
Pathways
GOT2 functions in the malate-aspartate shuttle, regulating cytosolic and mitochondrial NADH/NAD⁺ balance. It influences TCA cycle flux and amino acid metabolism, with upstream regulation by substrate availability and downstream effects on energy production.
For Research Use Only | Not For Clinical Use
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