CytoFinal™ Human Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB98
Product Type: ELISA Kit
Species: Human
Target: Human mitochondrial Aspartate Aminotransferase (GOT2)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies mitochondrial AST (GOT2) levels in human tissues, cell lysates, and biological fluids accurately.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit provides a high-precision in vitro solution for quantitative measurement of GOT2 concentrations. Ideal for analyzing tissue homogenates, cell lysates, and other biological samples, this kit enables reproducible assessment of mitochondrial enzymatic activity in human research models.
Features
Sandwich ELISA format offers high specificity and reproducibility for human GOT2 detection in diverse biological samples.
Colorimetric readout ensures straightforward, sensitive quantification across a wide concentration range (0.78-50 ng/mL).
Lyophilized standards and reagents provide long-term stability, minimizing activity loss and performance fluctuations.
Comprehensive 96-well kit accommodates high-throughput analysis while maintaining assay consistency and ease of use.
Process Relevance
Monitors mitochondrial AST activity to support metabolic research and biomarker evaluation in human samples.
Application Stage
Drug discovery, mechanistic studies, and in-process biomarker validation.
Applications
Facilitates quantitative GOT2 measurement in ELISA assays using tissue homogenates, cell lysates, serum, or other biological fluids for downstream ADME profiling studies.
Target
Human mitochondrial Aspartate Aminotransferase (GOT2)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Mitochondrial metabolism, liver disease research, cardiovascular studies, neurodegeneration, biomarker discovery, pharmacokinetics (ADME), metabolic syndrome, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement using colorimetric detection.
Assay Range
0.78 ng/ml - 50 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GOT2 is a mitochondrial enzyme critical for the malate-aspartate shuttle and amino acid metabolism. Accurate measurement of its activity provides insights into energy metabolism, liver function, and cellular stress responses, making it a vital biomarker in human studies.
Pathways
GOT2 participates in the malate-aspartate shuttle, transferring amino groups between glutamate and oxaloacetate. It influences TCA cycle flux, NADH/NAD+ balance, and downstream energy metabolism in mitochondria. Altered GOT2 activity impacts amino acid homeostasis and metabolic regulation.
For Research Use Only | Not For Clinical Use
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