CytoFinal™ Rat L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit

CAT#: ITS-0326-WB126
Product Type: ELISA Kit
Species: Rat
Target: Rat lactate dehydrogenase A chain
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures rat LDHA protein levels in biological samples using a sensitive ELISA assay.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit is a highly sensitive sandwich ELISA designed for the quantitative detection of LDHA in rat biological samples. This assay enables accurate measurement of LDHA levels in serum, plasma, tissue homogenates, and cultured cell samples, supporting investigations into cellular metabolism, hypoxia responses, and disease-associated metabolic alterations.
Features
Sensitive sandwich ELISA platform enables reliable quantification of rat LDHA across a broad concentration range in multiple biological matrices.
Carefully optimized antibody pairs provide strong specificity for rat LDHA with minimal cross-reactivity and low background signal.
Pre-coated microplate and validated reagents simplify experimental workflows while maintaining high reproducibility across independent assays.
Compatible with various rat sample types including serum, plasma, tissue extracts, and cultured cell lysates.
Process Relevance
Quantifies LDHA expression to evaluate glycolytic activity and metabolic alterations in rat experimental models.
Application Stage
Basic research, metabolic pathway studies, disease modeling, and preclinical drug evaluation.
Applications
Quantitative measurement of rat LDHA in serum, plasma, tissue lysates, or cultured cells for metabolic analysis, cytotoxicity evaluation, biomarker studies, ADME profiling studies, and glycolysis pathway research.
Target
Rat lactate dehydrogenase A chain
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic biology, glycolysis regulation, cancer metabolism, pharmacokinetics (ADME), hypoxia research, inflammation biology, toxicology, biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using absorbance-based endpoint ELISA detection.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.61 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) is a key enzyme in anaerobic glycolysis that catalyzes the conversion of pyruvate to lactate while regenerating NAD+. This reaction sustains cellular energy production under hypoxic or metabolically demanding conditions. Altered LDHA expression is frequently associated with metabolic reprogramming, inflammation, and tissue injury.
Pathways
LDHA functions within the glycolysis and lactate fermentation pathways, supporting NAD+ regeneration required for sustained glycolytic flux. Its expression is commonly regulated by hypoxia-responsive transcription factors such as HIF-1α. Increased LDHA activity contributes to metabolic adaptation in proliferating and stressed cells.
For Research Use Only | Not For Clinical Use
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