CytoFinal™ Low Sample Volume Rat Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB79
Product Type: ELISA Kit
Species: Rat
Target: Rat alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
High-sensitivity ELISA kit for measuring ALT1 levels in minimal rat serum, plasma, tissue, or cell lysate samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Rat Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit enables precise detection of ALT1 in rat biological samples using minimal volumes. The kit employs a sandwich ELISA format with high-affinity antibodies, supporting reliable hepatocellular function assessment and preclinical liver toxicity evaluation in small-volume samples.
Features
Minimal sample requirement: Accurately quantifies ALT1 using microliter-scale samples, conserving valuable experimental material.
High sensitivity and specificity: Detects ALT1 with negligible cross-reactivity to other aminotransferase isoforms.
Convenient microplate format: Standard 96-well plate compatible with common ELISA readers for high-throughput analysis.
Complete kit components: Includes ready-to-use standards, buffers, and detection reagents for consistent and reproducible results.
Process Relevance
Monitors ALT1 activity in rats, enabling early detection of hepatocellular stress and liver dysfunction during preclinical studies.
Application Stage
Preclinical research, drug safety evaluation, liver toxicity screening, and translational biomarker studies.
Applications
Quantifies ALT1 in rat serum, plasma, tissue, and cell lysates for hepatotoxicity studies, metabolic pathway analysis, ADME profiling studies, and preclinical liver disease research.
Target
Rat alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, toxicology, pharmacology, metabolic disease research, pharmacokinetics (ADME), preclinical safety assessment, biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative enzyme protein measurement via sandwich ELISA absorbance readout.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.31 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT1 catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, forming pyruvate and glutamate. Its activity reflects hepatocellular integrity and overall liver metabolic health in preclinical rat models.
Pathways
ALT1 participates in the alanine cycle, linking amino acid catabolism to gluconeogenesis. Pyruvate feeds into the TCA cycle, integrating nitrogen metabolism with energy production and cellular homeostasis.
For Research Use Only | Not For Clinical Use
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