CytoFinal™ Mouse L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit

CAT#: ITS-0326-WB123
Product Type: ELISA Kit
Species: Mouse
Target: Mouse lactate dehydrogenase A chain
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures mouse LDHA protein levels in biological samples using a sandwich ELISA format.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Mouse L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit is a highly sensitive sandwich ELISA designed for the quantitative detection of LDHA in mouse biological samples. The kit enables accurate measurement of LDHA concentrations in serum, plasma, tissue homogenates, or cell culture samples, supporting investigations into cellular metabolism, hypoxia responses, and disease-associated metabolic alterations.
Features
Highly sensitive sandwich ELISA format enables reliable quantification of mouse LDHA across a wide dynamic concentration range.
Carefully optimized antibody pairs provide strong specificity for mouse LDHA while minimizing background interference and cross-reactivity.
Standardized reagents and streamlined workflow support reproducible results and efficient processing in routine laboratory assays.
Compatible with diverse biological samples including mouse serum, plasma, tissue lysates, and cultured cell supernatants.
Process Relevance
Quantifies LDHA to monitor glycolytic metabolism changes and cellular stress responses in experimental models.
Application Stage
Basic research, drug discovery, metabolic pathway studies, and experimental disease model analysis.
Applications
Detection of mouse LDHA levels in serum, plasma, tissue extracts, or cultured cells for metabolic studies, cytotoxicity evaluation, disease biomarker analysis, ADME profiling studies, and glycolysis pathway research.
Target
Mouse lactate dehydrogenase A chain
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic biology, glycolysis regulation, cancer metabolism, pharmacokinetics (ADME), hypoxia biology, toxicology, inflammation research, biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using absorbance-based endpoint ELISA detection.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.62 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) is a central metabolic enzyme that catalyzes the conversion of pyruvate to lactate while regenerating NAD+. This reaction supports sustained glycolysis under hypoxic or high metabolic demand conditions. Dysregulated LDHA expression is frequently associated with tumor metabolism, inflammation, and cellular injury.
Pathways
LDHA participates in the glycolytic pathway and lactate fermentation, maintaining NAD+ availability for continuous glycolytic flux. Its expression is commonly regulated by hypoxia-responsive transcription factors such as HIF-1α. Elevated LDHA activity contributes to metabolic reprogramming observed in cancer and rapidly proliferating cells.
For Research Use Only | Not For Clinical Use
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