MetaVital™ Mouse Dimethylaniline monooxygenase [N-oxide-forming] 3 (FMO3) ELISA Kit

CAT#: ITS-0326-WB23
Product Type: ELISA Kit
Species: Mouse
Target: Mouse Dimethylaniline Monooxygenase [N-oxide-forming] 3 (FMO3)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies FMO3 protein levels in mouse tissues, cell lysates, and biological fluids using a sensitive ELISA platform.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ FMO3 ELISA Kit provides precise in vitro quantification of Mouse Dimethylaniline Monooxygenase [N-oxide-forming] 3 in tissue homogenates, cell lysates, and biological fluids. This high-sensitivity colorimetric assay delivers reproducible measurements across a broad dynamic range, facilitating research in xenobiotic metabolism and enzymatic activity.
Features
Highly specific antibodies ensure accurate detection of mouse FMO3 with minimal cross-reactivity among related FMO isoforms.
Colorimetric detection enables quantification from 0.156 ng/mL to 10 ng/mL, supporting reliable experimental reproducibility.
Lyophilized reagents and standards enhance long-term stability and consistent performance across multiple assays.
Compatible with tissue homogenates, serum, plasma, and cell lysates, offering flexibility for diverse sample types.
Process Relevance
Enables accurate monitoring of FMO3 expression to support drug metabolism and toxicological research in mouse models.
Application Stage
Drug discovery, metabolic pathway investigation, and in vitro biomarker analysis.
Applications
Quantitative ELISA assessment of FMO3 in mouse tissues, serum, plasma, and cell lysates for pharmacological, ADME profiling studies, and metabolic studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Mouse Dimethylaniline Monooxygenase [N-oxide-forming] 3 (FMO3)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Xenobiotic metabolism, pharmacokinetics (ADME), toxicology, drug development, enzymology, metabolic pathway analysis, mouse liver biology, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric endpoint detection.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
FMO3 is a flavin-containing monooxygenase involved in N-oxidation of xenobiotics and endogenous amines in mice. Its measurement is crucial for understanding species-specific metabolism, drug clearance, and metabolic regulation.
Pathways
FMO3 functions in xenobiotic biotransformation, catalyzing N-oxidation of amines. Its activity influences downstream metabolite formation, detoxification, and hepatic metabolic regulation in mice.
For Research Use Only | Not For Clinical Use
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