Dog HMGB1 ELISA Kit, Lot 21AO-426 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P337)

Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip

0.078 ng/mL

100 μL

1 - 4.5 h

Pre-coated

Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.

Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

4 °C,-20 °C

Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.

6 months

Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.

For Research Use only

High Mobility Group Protein 1 (HMGB1)

HMG1; HMG3; SBP-1; DEF; HMG-1; Hmg1; amphoterin; p30; hmgb1; ik:tdsubc_1a5; wu:fb23c02; xx:tdsubc_1a5; zgc:56110; zgc:77104; hmg-1; hmg3; sbp-1; hmg1; HMGB1; Ac2-008; high mobility group box 1; high-mobility group box 1; high mobility group box 1a; high mobility group box 1 L homeolog; high mobility group protein B1; HMGB1; Hmgb1; hmgb1; hmgb1a; hmgb1.L; LOC100359149

Abbreviation: HMGB1

Q6YKA4

p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome