Dog HMGB1 ELISA Kit, Lot 21AO-426 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P337
Product Type: ELISA Kit
Target: HMGB1
Short Description
The kit is designed for in vitro quantitative measurement of Dog HMGB1 in Cell Lysate, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of dog high mobility group protein B1 (HMGB1) concentrations in serum, plasma, tissue homogenates, cell lysates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
HMGB1
Reactivity
Dog
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Lysate, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of dog HMGB1. No significant cross-reactivity or interference between dog HMGB1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between dog HMGB1 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
0.078 ng/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.
Restrictions
For Research Use only
Alternative Name
High Mobility Group Protein 1 (HMGB1)
Synonyms
HMG1; HMG3; SBP-1; DEF; HMG-1; Hmg1; amphoterin; p30; hmgb1; ik:tdsubc_1a5; wu:fb23c02; xx:tdsubc_1a5; zgc:56110; zgc:77104; hmg-1; hmg3; sbp-1; hmg1; HMGB1; Ac2-008; high mobility group box 1; high-mobility group box 1; high mobility group box 1a; high mobility group box 1 L homeolog; high mobility group protein B1; HMGB1; Hmgb1; hmgb1; hmgb1a; hmgb1.L; LOC100359149
Background
Abbreviation: HMGB1
UniProt
Q6YKA4
Pathways
p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
For Research Use Only | Not For Clinical Use
Online Inquiry