Deubiquitinase (DUB) Panel Screening Service
Our Deubiquitinase (DUB) Panel Screening Service provides a powerful platform for identifying and characterizing inhibitors of deubiquitinase enzymes. DUBs play a pivotal role in regulating protein degradation, signaling pathways, and various cellular processes through the removal of ubiquitin molecules from target proteins. Many illnesses, including as cancer, neurological conditions, and immune system malfunction, have been connected to the dysregulation of DUBs. By leveraging our advanced high-throughput screening technology, researchers can efficiently evaluate compounds for their potential to modulate DUB activity, facilitating drug discovery and therapeutic development.
Service Background
Ubiquitination and deubiquitination are essential post-translational modifications that control protein stability, localization, and function. Ubiquitin is conjugated to proteins in a process called ubiquitination, marking them for degradation via the proteasome. Conversely, DUBs cleave ubiquitin chains from proteins, regulating protein homeostasis and affecting processes such as cell cycle control, DNA repair, and immune responses.
Fig.1 The cascades of ubiquitination/deubiquitination and families of DUBs.1
DUBs are categorized into several families, each with distinct structural and functional properties. They are involved in the regulation of key pathways that govern disease progression, making them attractive targets for therapeutic intervention. Our DUB Panel Screening Service enables researchers to systematically screen compounds or biological samples for their ability to modulate DUB activity, contributing to the development of targeted therapies for diseases associated with ubiquitin dysregulation.
Deubiquitinase (DUB) Panel Screening Service at Creative Biolabs
We offer:
Our ATPase Panel Screening Service includes a comprehensive suite of assays designed to measure the activity of various ATPases. The service encompasses:
Our DUB Panel Screening Service offers a comprehensive screening platform for a variety of deubiquitinase enzymes, including but not limited to:
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UCHL1 – A crucial DUB involved in maintaining cellular protein turnover and regulation.
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USP7 (HAUSP) – A well-known DUB that plays a significant role in regulating the p53 tumor suppressor and various cellular signaling pathways.
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OTU DUBs: This family of DUBs is essential for controlling inflammation and immunological responses.
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Ape1/2 – DUBs that are essential for DNA repair mechanisms.
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USP14 – A DUB associated with the regulation of proteasomal activity.
We offer two primary types of assays:
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Activity Assays: To evaluate the enzymatic activity of DUBs in the presence of substrates and inhibitors.
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Inhibition Assays: To assess the ability of compounds to inhibit DUB activity, providing insight into their potential therapeutic applications.
Service process:
Why Choose Us
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Comprehensive DUB Panel: Our service includes a wide range of DUBs, allowing for the evaluation of different DUB families and their involvement in diverse biological processes.
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High-Throughput Screening: We employ state-of-the-art technologies and automated systems to conduct large-scale screening, saving time while maximizing the accuracy and reproducibility of results.
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Customizable Assays: The screening panel can be customized to focus on specific DUBs or experimental conditions tailored to your research objectives.
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Data Transparency: Detailed analysis of screening results, including dose-response curves, IC50 calculations, and compound profiling, provides clear insights into the effects of each compound.
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Professional Advice: To assist with result interpretation, follow-up experiment design, and compound selection optimization, our skilled staff provides individualized support.
Frequently Asked Questions
A1: Which DUBs are included in the screening panel?
Q1: Our panel includes a broad range of DUBs such as UCHL1, USP7, OTU DUBs, Ape1/2, and USP14. On request, more DUBs can be added.
A2: How can you guarantee the caliber of your output?
Q2: All assays are validated using positive and negative controls, and each experiment is conducted in triplicate to ensure reliability and reproducibility. We use advanced technologies like mass spectrometry and fluorescence for precise measurements.
Contact Us
To learn more about our Deubiquitinase (DUB) Panel Screening Service or discuss your specific research needs, please feel free to contact us. Our team of professionals is here to assist you in navigating the difficulties of drug development and research linked to DUB. We look forward to partnering with you to advance your scientific discoveries and therapeutic innovations!
Reference
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Zhou, Zhuan et al. "The Emerging Role of Deubiquitinases in Cell Death." Biomolecules vol. 12,12 1825. 6 Dec. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only | Not For Clinical Use
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