Creative Biolabs provides bioassay and multi-omics services to measure system immunity in gut microbiota-associated neuro diseases, based on the interplay between the gut microbiome and the immune system.
Learn More →Embryonic Stem Cell (ESC) Immunophenotyping Service
Creative Biolabs' advanced immunophenotyping panels are designed to provide a complete profile of your embryonic stem cells (ESCs). We meticulously screen for the presence of key pluripotency markers that define the undifferentiated state of ESCs, including TRA-1-60, SSEA-3, SSEA-4, TRA-1-81, and the nuclear transcription factors OCT4, SOX2, and NANOG. The expression of these markers provides definitive evidence that your cell line has maintained its pluripotent potential.
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ESC Immunophenotyping: Ensuring Consistency and Quality for Your Research
The field of regenerative medicine is being revolutionized by the unique properties of ESCs. Their unparalleled potential for pluripotency—the ability to differentiate into any cell type of the three primary germ layers—makes them invaluable for therapeutic development, disease modeling, and drug discovery. However, the immense promise of ESCs is entirely contingent on the ability to maintain and verify their undifferentiated state and genetic stability. This is where the power of immunophenotyping becomes indispensable. As a core service at Creative Biolabs, our comprehensive ESC immunophenotyping service provides the critical data needed to ensure the identity, purity, and quality of your cell lines. We leverage our two decades of experience and state-of-the-art technology to offer a level of characterization that is both rigorous and reliable.
Fig.1 Major types of ESC markers. 1
Unparalleled Expertise and Advanced Technology
At Creative Biolabs, our immunophenotyping service goes beyond simple marker detection.
Multi-parameter Flow Cytometry
We use this technology for simultaneous, high-precision detection of multiple markers on single cells.
Comprehensive Analysis
The high-throughput approach provides a quantitative analysis of cell populations, offering deep insights into heterogeneity.
High-Resolution Immunofluorescence Microscopy
This is used for visual confirmation of marker expression and cellular morphology.
Rigorous Analytical Approach
Our expert scientists combine these technologies to provide detailed, easily interpretable reports with actionable data.
Discover How We Can Help - Request a consultation to discuss the specific needs of your project.
Why Choose Us?
Choosing the right partner for stem cell characterization is crucial. Creative Biolabs leverages over two decades of experience, offering expertise that goes beyond basic analysis. We utilize a sophisticated, multi-marker approach to overcome the challenge of marker overlap with tumor stem cells, a crucial consideration for clinical applications. Our service is also capable of detecting subtle issues like blastomere mosaicism, which can compromise research integrity. We provide a rigorous and reliable level of characterization to ensure the identity, purity, and quality of your cell lines.
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FAQs
Q1: Why do I need to perform immunophenotyping on my ESCs if they look healthy under a microscope?
A1: While visual inspection is a good starting point, it cannot reveal the molecular characteristics of your cells. Immunophenotyping provides a quantitative, objective measure of pluripotency and purity, detecting subtle but critical issues like early differentiation or contamination that are invisible to the naked eye.
Q2: What specific markers do you test for?
A2: Our standard panels include key pluripotency markers like TRA-1-60, SSEA-3, SSEA-4, and the nuclear factors OCT4 and SOX2. We also use a panel of negative markers, such as TROMA-1, to confirm the absence of differentiated cells.
Q3: My cell line is a newly established one. Can you assist me in characterizing it?
A3: Absolutely. Our service is ideal for validating newly established cell lines. We can provide a complete characterization to confirm its identity, pluripotency, and stability, giving you a solid foundation to build your research upon.
Customer Review
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Reliable Quality Control
Using Creative Biolabs' service in our research has significantly improved batch-to-batch consistency. The detailed report and quantitative data gave us confidence in our findings, which is crucial for our publication and grant applications. - N***an -
Tangible Benefits
We initially relied on a limited marker panel, but Creative Biolabs' comprehensive service identified a potential marker overlap with tumor stem cells that we would have missed. This insight prevented a major setback in our pre-clinical work and saved us considerable time and resources. - L***lie
Related Services
To complement your ESC immunophenotyping project, we recommend exploring our other services:
System Immunity Measurement
Immuno-oncology Phospho-flow Cytometry Service
Creative Biolabs offers immuno-oncology phospho-flow cytometry service. We combine flow cytometry with phospho-specific antibodies to analyze the phosphorylation status of signaling molecules, providing unparalleled insights for targeted cancer therapies.
Learn More →How to Contact Us
Creative Biolabs is your trusted partner for high-quality stem cell research. Our dedicated team is ready to provide the expertise and cutting-edge technology you need to ensure the success and integrity of your projects.
Contact Our Team for More Information and to Discuss Your Project
Reference
- Zhao, Wenxiu, et al. "Embryonic stem cell markers." Molecules 17.6 (2012): 6196-6246. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/molecules17066196
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