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IntroductionMicroarray vs. RNA-seqAnalysis PipelinePublicationFAQsCustomer ReviewRelated ServicesContact UsBrochure
Gene expression profiling is a powerful technique used to analyze gene activity across thousands of cells simultaneously, providing a global view of cellular function. This method allows for the identification of
new drug targets by comparing gene expression between treated and untreated cells, facilitating the evaluation of treatment-related phenotypic changes at the molecular level. Gene expression profiling is particularly valuable for studying
the tumor microenvironment, identifying target cells, and advancing drug discovery.
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Creative Biolabs offers comprehensive gene expression profiling services, leveraging extensive expertise across molecular biology, genetics, and bioinformatics. Our team is proficient in advanced techniques such as DNA microarrays, transcriptomics sequencing, digital PCR, and real-time PCR, enabling us to provide customized solutions tailored to each client's project needs. We focus on selecting and optimizing the most appropriate technology for
each research goal, ensuring accurate and high-quality results.
Microarray Analysis vs. RNA-seq Analysis
Aspect
Microarray Analysis
RNA-seq Analysis
Technology
hybridization-based techniques
predefined probes
sequencing technology
capture and quantify RNA transcripts
Platforms
Affymetrix - a pioneer in microarray technology
Agilent - an integrated, flexible, open approach
Illumina - a highly reproducible, multiplex efficient, and cost-effective option
Next-generation sequencing (NGS)
Single cell sequencing
In situ sequencing of fixed tissue
Applications
Suitable forgene expression studies that focus on:
Well-annotated genomes
Predefined targets
Suitable for discovering novel genes:
Novel genes discovery
Alternative splicing events
Entire transcriptomes analysis
Scope of Analysis
Narrow, limited to known genes or transcriptsthatpresent in the microarray probes
Broad, both known and novel transcripts, including isoforms and alternative splicing
Data Output
(Whether predefined probes are required?)
Yes, it provides relative gene expression levels based on predefined probes
No, it offers absolute quantification of RNA transcripts without relying on predefined probes.
Sensitivity
Suitable for high-abundance transcripts
High sensitivity forlow-abundance transcripts andnovel RNA species
Microarray analysis and RNA-seq analysis can be applied to a wide range of organisms, including humans, mice, and rats. Microarray analysis is ideal for quick and cost-effective studies where the focus is on known gene expression
profiles, while RNA-seq analysis offers deeper insights, detecting novel transcripts and providing base-pair resolution, making it suitable for more comprehensive and exploratory studies. Creative Biolabsis professional in recommending the best-fit solutions according to your specific requirements. If you hesitate about choosing the more suitable method and want to get more customized suggestions for your research, please feel free to contact us.
Analysis Pipeline
Array Data Extraction
↓Detectable Signal
Data Normalization
↓Quantile
Statistical Testing
↓P-value Calculation
RNA-seq Analysis
↓Test Enrichment calculation
Final Report
Why Choose Us?
Applicable to various species
Tailored solutions for humans, mice, rats, and other model organisms.
Streamlined process
Standard genome-wide expression analysis for you.
Custom array design and validation
Tailored solutions from method determination to final analysis.
Professional support
Expert technical support throughout the project to ensure high repeatability, efficiency, and cost-effectiveness.
Publication
This publication details a collaborative study by the long-read RNA-seq genome annotation assessment project (LRGASP) consortium. The consortium evaluated long-read RNA sequencing approaches for transcriptome analysis, generating over 427 million long-read sequences from human, mouse, and manatee species using various protocols and platforms. The study aimed to address challenges in transcript isoform detection, quantification, and de novo transcript detection. Key findings indicate that longer, more accurate sequences yield more accurate transcripts, while greater read depth improves quantification accuracy. Tools based on reference sequences performed best in well-annotated genomes. This collaborative effort provides a benchmark for current practices and guides future method development in transcriptome analysis.
Fig.1 A Comprehensive data resource for the LRGASP consortium.1
FAQs
Q1: What types of samples can I submit for gene expression profiling?
A1: Creative Biolabs accepts a wide range of biological samples to accommodate diverse research needs. Our team provides detailed guidelines and support to ensure optimal sample preparation, which is crucial for maximizing data quality and the success of your project.
Q2: How do you ensure the accuracy and reliability of the gene expression data?
A2: At Creative Biolabs, we employ stringent quality control measures at every step of the gene expression profiling workflow. From initial sample assessment and meticulous RNA extraction to precise library preparation and high-throughput sequencing or hybridization, our processes are designed for accuracy.
Q3: Can your service help me identify novel biomarkers for my disease of interest?
A3: Absolutely! While proteins are indeed common therapeutic targets, Creative Biolabs' capabilities extend comprehensively to other emerging target classes, including crucial nucleic acids (such as mRNA), various lipids, and complex carbohydrates.
Customer Review
Unparalleled Precision
Using Creative Biolabs' RNA-seq service in our cancer research has significantly improved our ability to identify subtle but critical gene expression changes in tumor microenvironments. The data quality and the depth of bioinformatics analysis were exceptional, far surpassing our previous in-house efforts. - Dr. La Mn
Insightful Bioinformatics
Creative Biolabs' gene expression profiling service, particularly their machine learning integration, helped us uncover previously unrecognized gene signatures in autoimmune disease progression. The detailed reports and interactive visualizations provided actionable insights that directly guided our next experimental steps, saving us months of work. - Dr. JS Pl
Please feel free to contact us for more detailed information.
Related Services
To further support your research and development goals, Creative Biolabs offers a suite of complementary services that can be seamlessly integrated with our gene expression profiling offerings, providing a holistic view of biological systems:
Host Transcriptome Characterization
Host transcriptome characterization is vital for biomedical research, unraveling gene expression in health and disease, enabling precision medicine. Revolutionary single-cell and dual RNA sequencing provide high-resolution profiling for innovations in infectious disease, cancer, and immunology.
Creative Biolabs offers high-quality, customized RNA-based tumor cell test services and products at economical prices. Their professional team provides comprehensive project management, from consultation and design to progress updates and after-sales service, ensuring smooth project execution.
Ready to unlock the full potential of your biological research? Contact our team at Creative Biolabs today to discuss your specific project needs and learn how our gene expression profiling services can provide the precise insights you require to achieve your scientific objectives.
Contact Our Team for More Information and to Discuss Your Project. Inquire Now
Reference
Pardo-Palacios, Francisco J., et al. "Systematic assessment of long-read RNA-seq methods for transcript identification and quantification." Nature methods 21.7 (2024): 1349-1363. Distributed under Open Access license CC BY 4.0, without modification.
Fig.1 A Comprehensive data resource for the LRGASP consortium.1
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