Short Description
Measures reduced (GSH) and oxidized (GSSG) glutathione.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
The GuardianOx™ GSH/GSSG Assay Kit provides a quantitative measure of cellular redox status, essential for screening compounds that induce apoptosis or confer cytoprotection via antioxidant pathways. By determining the ratio of reduced to oxidized glutathione, it serves as a primary screen for oxidative stress induction. This assay is a cornerstone of mechanistic toxicology, helping to elucidate the mode of action for chemotherapeutic agents and cytoprotective drugs.
Features
Determines the ratio of reduced to oxidized glutathione; Primary screen for oxidative stress induction and antioxidant efficacy in cellular assays.
Process Relevance
Oxidative Stress Mechanism Study
Application Stage
Discovery / Mechanistic Tox
Applications
Critical indicator of oxidative stress and apoptosis research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Whole blood, plasma, serum, urine, tissue, and cell extracts
Research Areas
Oxidative Stress; Cell Biology
Datasheet