GuardianOx™ GSH/GSSG Assay Kit

CAT#: ITS-1125-HM29
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Measures reduced (GSH) and oxidized (GSSG) glutathione.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
The GuardianOx™ GSH/GSSG Assay Kit provides a quantitative measure of cellular redox status, essential for screening compounds that induce apoptosis or confer cytoprotection via antioxidant pathways. By determining the ratio of reduced to oxidized glutathione, it serves as a primary screen for oxidative stress induction. This assay is a cornerstone of mechanistic toxicology, helping to elucidate the mode of action for chemotherapeutic agents and cytoprotective drugs.
Features
Determines the ratio of reduced to oxidized glutathione; Primary screen for oxidative stress induction and antioxidant efficacy in cellular assays.
Process Relevance
Oxidative Stress Mechanism Study
Application Stage
Discovery / Mechanistic Tox
Applications
Critical indicator of oxidative stress and apoptosis research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Whole blood, plasma, serum, urine, tissue, and cell extracts
Research Areas
Oxidative Stress; Cell Biology
Size
100 tests
Data Output Type
Quantitative (Ratio)
Assay Time
Results in 20 min
LOD
10 nM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutathione exists in reduced (GSH) and oxidized (GSSG) states. The ratio of GSH to GSSG is a dynamic indicator of cellular oxidative stress and redox balance reflecting the cell's ability to detoxify reactive oxygen species.
For Research Use Only | Not For Clinical Use
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