Single-cell transcriptional analysis has already been used in hundreds of fruitful studies through high-throughput sequencing, which has made exciting new biological discoveries. Creative Biolabs offers gut microbiota single-cell transcriptional profiling to precisely and accurately reconstruct the composition of a functional biological unit at the highest resolution, elucidating the networking and dynamic interplay between individual cells.
The single-cell transcriptional profiling, also known as single-cell RNA sequencing (scRNAseq), has changed the field of immunology, revealing an unappreciated complexity of immune cell subsets, identifying new cell types and states, redefining cellular ontogeny and enabling inference of cell fate trajectories and function. ScRNAseq can piece together existing knowledge of cell markers, ontology and interactions into an integrative picture of the building blocks of human tissues. Besides, scRNAseq allows for the hypothesis-generating phase of research and be guided by tissue-specific clues. Targeted experiments in model systems can then be used to support findings and test biological mechanisms.
Typical single-cell transcriptional protocol consists of three major modular steps: double-stranded complementary DNA (cDNA) synthesis, cDNA amplification and sequencing library preparation (shown in Fig.1).
Fig.1 Single-cell transcriptional experimental pipeline. (Wu, 2017)
Fig.2 ScRNAseq approaches and analysis tools for studying intestinal immunity. (James, 2021)
With a team of microbiome experts in gut microbiota single-cell transcriptional profiling and complex data analysis, Creative Biolabs provides comprehensive and professional Gut Microbial Biomarker Discovery Services to meet all the needs of our customers. If you are interested in our service, please feel free to contact us for any discussion.
References
For Research Use Only | Not For Clinical Use