Introduction

Hepatocellular carcinoma (HCC) is a major cause of cancer-related death worldwide, posing significant challenges in terms of treatment efficacy and disease recurrence. Hep 3B cells, a human liver carcinoma cell line, are extensively used in cell-based assays for cancer research due to their stable proliferation and tumorigenic properties. At Creative Biolabs, we have developed a suite of advanced Hep 3B-based proliferation assays that integrate cutting-edge luminescence technology to monitor cellular responses under various experimental conditions.

Hep 3B-Based Proliferation Assay at Creative Biolabs

Creative Biolabs leverages the unique biological characteristics of Hep 3B cells to design robust proliferation assays. One key feature of our Hep 3B-based assays is their sensitivity and specificity in detecting cell viability and proliferation, which are critical for evaluating the efficacy and toxicity of anticancer compounds. Using proprietary methodologies, we ensure accurate and reproducible measurements of cellular responses.

Cell-Based Proliferation Assays Customized to Customers' Program

At Creative Biolabs, we understand that each research program has unique needs. Our expertise in assay customization allows us to develop fit-for-purpose proliferation assays tailored to the specific requirements of our clients. Our cell-based proliferation assays encompass both 2D and 3D culture formats using Hep 3B cells.

2D Assay Formats

Multi-well Plates: Standard format for high-throughput screening, facilitating parallel testing of multiple compounds.

Transwells: Advanced format enabling co-culture and barrier permeability studies.

3D Assay Formats

Air-Liquid Interface: Mimics the in vivo environment more closely than 2D cultures.

Matrix-Based and Matrix-Free Systems: Provide insights into the behavior of Hep 3B cells in various extracellular matrices.

Simple or Co-Culture Systems: Enable the study of interactions between Hep 3B cells and other cell types.

Our breadth of analytical platforms allows us to deliver comprehensive assay endpoints, including proliferation marker detection, gene expression alterations, and real-time cellular imaging, thereby providing a holistic view of cellular responses.

User-Friendly Luminescence-Based Proliferation Assay

Creative Biolabs offers a bioluminescence-based proliferation assay that accurately and efficiently measures Hep 3B cell viability in a high-throughput mode. Our Hep 3B proliferation assays utilize the principle of ATP-dependent luminescence, where metabolically active cells produce ATP that fuels the luciferase reaction, resulting in light emission. The intensity of the luminescence correlates with the number of viable cells, thereby providing a direct readout of cell proliferation. This assay uses a simplified add-and-read format, tailored for optimal performance with the standard luminometer.

Key Parameters

Detection Method: Luminescence

Assay Type: Functional, Cell-Based

Functional Mode: Antagonist

Test Concentration/Dose: Compounds screened at different concentrations, generally ranging from 0.01 µM to 30 µM

Measured Response: Luminescence indicating cell viability and proliferation

Controls: Staurosporine as a positive control for growth inhibition

Scientific Backing

Human liver cell Hep 3B-based proliferation assay has been used in the development of many anticancer drugs and the study of related signaling pathways.

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Frequently Asked Questions

Q1: Can the assays be customized for specific research needs?

A1: Absolutely. We specialize in developing fit-for-purpose assays tailored to meet the unique requirements of each client's research program. Our customization options include different culture formats and multiple assay endpoints.

Q2: What type of support can clients expect from Creative Biolabs?

A2: We offer comprehensive support, including project consultation, project development, data analysis, and troubleshooting. Our experienced experts are always available to assist you throughout your research journey.

Creative Biolabs excels in providing innovative Hep 3B-based proliferation assays designed to meet the evolving needs of cancer research and drug discovery. Our commitment to scientific excellence, coupled with our customized approach, ensures that we deliver high-quality and reliable data, driving your research forward.

Reference

1. Kim, So Young, et al. "Induction of apoptosis by coptisine in Hep3B hepatocellular carcinoma cells through activation of the ROS-mediated JNK signaling pathway." International Journal of Molecular Sciences 21.15 (2020): 5502. Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use