Hep 3B In Vitro BrdU Assay (Cell Proliferation)

CAT#: ITS-1022-YF2114
Target Cell Organism: Human
Target Cell Alternative Name: Hep-3B; Hep3B; Hep 3B2.1-7
Target Cell Name: Hep 3B
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Hep 3B-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Hep 3B
Target Cell Organism
Human
Target Cell Background
Hep 3B is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.
Target Cell Alternative Name
Hep-3B; Hep3B; Hep 3B2.1-7
Related Diseases
Hepatocellular Carcinoma
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Short Description
Hep 3B-cell based In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent signal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Proliferation Assay
Assay MOA
Cell-based Assays Based on DNA Synthesis
For Research Use Only | Not For Clinical Use
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