Human BCMA Sandwich ELISA Kit-48 pg/mL

This product is a 96-well enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of human BCMA in samples of cell culture, supernatants, plasma, serum.

BCMA detection

BCMA

Human

Colorimetric

Quantitative

Cell culture, supernatants, plasma, serum

96-well microplate

TNF receptor superfamily

Certificate of quality

Pre-coated 96-well strip microplate; Wash buffer; Stop solution; Assay diluent(s); Lyophilized standard; Biotinylated detection antibody; Streptavidin-conjugated HRP; TMB one-step substrate

48 pg/mL

0.048 ng/mL-12 ng/mL

1, 2, or 5

1.Distilled or deionized water 2.Precision pipettes to deliver 2 µL to 1 µL volumes 3.Adjustable 1-25 µL pipettes for reagent preparation 4.100 µL and 1 liter graduated cylinders 5.Tubes to prepare standard and sample dilutions 6.Absorbent paper 7.Microplate reader capable of measuring absorbance at 450nm 8.Log-log graph paper or computer and software for ELISA data analysis

1.Prepare all reagents, samples and standards as instructed in the manual; 2.Add 100 µL of standard or sample to each well; 3.Incubate 2.5 h at RT or O/N at 4°C; 4.Add 100 µL of prepared biotin antibody to each well; 5.Incubate 1 h at RT; 6.Add 100 µL of prepared Streptavidin solution to each well; 7.Incubate 45 min at RT; 8.Add 100 µL of TMB One-Step Substrate Reagent to each well; 9.Incubate 30 min at RT; 10.Add 50 µL of Stop Solution to each well; 11.Read at 450 nm immediately.

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.

Q02223

Stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage: -80°C.

For research use only. Intended for use by laboratory professionals.

TNFRSF17; B cell maturation antigen; BCMA; CD269 antigen; B-cell maturation factor; B-cell maturation protein; BCM; CD269

TNFRSF17; B cell maturation antigen; BCMA; CD269 antigen; B-cell maturation factor; B-cell maturation protein; BCM; CD269

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.

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