Human IL1F6 ELISA Kit, Lot 21AO-1266 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1177
Product Type: ELISA Kit
Target: IL1F6
Short Description
The kit is designed for in vitro quantitative measurement of Human IL1F6 in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human interleukin-36 alpha (IL1F6) concentrations in serum, plasma, tissue homogenates, cell culture supernates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
IL1F6
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human IL1F6. No significant cross-reactivity or interference between human IL1F6 and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IL1F6 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
3.9 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C.
Restrictions
For Research Use only
Alternative Name
interleukin 1 family, member 6 (epsilon)
Synonyms
Il1f6; Fil1; IL-1H1; IL1RP2; Il1f9; Il36a; FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1(EPSILON); IL1F6; interleukin 36; alpha; interleukin 1 family; member 6 (epsilon); interleukin 1 family; member 6; interleukin 36 alpha; Il36a; IL1F6; IL36A; Il1f6
Background
Abbreviation: IL36A
Alias: FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), MGC129552, MGC129553, IL-1F6 (FIL-1-epsilon)|interleukin 1, epsilon
Gene ID
27179
UniProt
Q9UHA7
Pathways
Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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