Human IL6R ELISA Kit, Lot 21AO-1355 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P1266)

Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Sample/Standard Dilution Buffer
Biotin-labeled Antibody (Concentrated)
Antibody Dilution Buffer
HRP-Streptavidin Conjugate (SABC)
SABC Dilution Buffer
TMB Substrate
Stop Solution
Wash Buffer (25 x concentrate)
Instruction manual

18.75 pg/mL

100 μL

3.5 h

Pre-coated

1.Bring all reagents to room temperature (18-25 °C) before use.
2.Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.
3.Standard working solution: Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 200pg/mL.
4.Biotinylated Detection Ab working solution: Centrifuge the Concentrated Biotinylated Detection Ab at 800xg for 1 min, then dilute the 100x Concentrated Biotinylated Detection Ab to 1x working solution with Biotinylated Detection Ab Diluent.
5.HRP Conjugate working solution: Calculate the required amount before the experiment.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-6R were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-6R were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

4 °C,-20 °C

6 months

For Research Use only

IL-6 Receptor (IL6R)

IL6R; il-6ra; CD126; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ; gp80; IL6R1; Il6ra; IL-6Ralpha; IL-6R; Il6r; interleukin 6 receptor; interleukin 6 receptor; alpha; IL6R; il6r; Il6r; Il6ra

3711

P08887

JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37 °C.
2.Remove the liquid. Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37 °C.
3.Aspirate and wash 3 times.
4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37 °C.
5.Aspirate and wash 5 times.
6.Add 90 μL Substrate Reagent. Incubate for 15 min at 37 °C.
7.Add 50 μL Stop Solution. Read at 450 nm immediately.