Human Phospho-EGFR (Tyr1086) ELISA Kit (CAT#: TM-HMM059)

Wash buffer; Cell lysis buffer; Assay diluent; HRP-streptavidin concentrate; Anti-phospho-EGFR; Biotinylated-anti-EGFR; EGFR microplate coated with monoclonal anti-EGFR antibody; HRP-conjugated anti-rabbit IgG; Positive control; Stop solution; TMB one-step substrate reagent

5h

An anti-EGFR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phosphorylated EGFR (Y1086) or anti-total EGFR antibody is used to detect phosphorylated or non-phosphorylated EGFR. After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG or HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR (Y1086) or total EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.

-20°C

For research use only. Intended for use by laboratory professionals.

EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1

EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1

The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.