Human Phospho-ERBB3/HER3 (Tyr1262) ELISA Kit

CAT#: TM-HMM231
Product Type: Cell-based phoshorylation ELISA kit
Target: ERBB3/HER3
Reactivity: Human
Applications: ERBB3/HER3 detection
Description
This product is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates.
Features
This assay semi-quantitatively measures phosphorylated ErbB3 (Tyr1262) in lysate samples.
Applications
ERBB3/HER3 detection
Target
ERBB3/HER3
Reactivity
Human
Detection Method
Colorimetric
Method Type
Semi-quantitative
Sample Type
Cell lysates, tissue lysates
Size
96-well plates
Receptor Family
Epidermal growth factor receptor (EGFR/ERBB) family
Qualified With
Certificate of quality
Components
Pre-coated 96-well strip microplate; Wash buffer; Anti-phospho antibody; HRP-conjugated secondary antibody; Assay diluent; TMB one-step substrate; Stop solution; Lysis buffer; Positive control sample
Plate
1, 2, or 5
Reagent Preparation
Distilled or deionized water; 100 mL and 1 liter graduated cylinders; Tubes to prepare sample dilutions; Protease and Phosphatase inhibitors; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; Benchtop rocker or shaker; Microplate reader capable of measuring absorbance at 450 nm.
Assay Procedure
Prepare all reagents and samples as instructed in the manual; Add 100 µL of sample or positive control to each well; Incubate 2.5 h at RT or O/N at 4 °C; Add 100 µL of prepared primary antibody to each well; Incubate 1 h at RT; Add 100 µL of prepared 1X HRP-Streptavidin to each well; Incubate 1 h at RT; Add 100 µL of TMB One-Step Substrate Reagent to each well; Incubate 30 min at RT; Add 50 µL of Stop Solution to each well; Read at 450 nm immediately.
Target/Description
Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.
Accession Number
P21860
Storage
-20°C
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3
Synonyms
ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3
Background
Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.
Gene ID
2065
For Research Use Only | Not For Clinical Use
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