PharmaOnco™ Optimal Methylcellulose Medium

Creative Biolabs has developed a series of mediums and supplements for cell culture, expansion and differentiation. Methylcellulose-based medium is optimized for the detection and quantification of human hematopoietic progenitor cells in bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples using colony-forming unit (CFU) assays. It is suitable for use with CD34+ enriched cells, mononuclear cells, and cells isolated by other purification methods. This medium has been formulated to support optimal growth of erythroid progenitor cells, granulocyte-macrophage progenitor cells, and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells.

Cell Culture, Colony Assay, Functional Assay

24 x 3 mL,
100 mL

Hematopoietic Stem and Progenitor Cells

Human, Non-Human Primate

Semi-Solid Media, Specialized Media

Drug Discovery and Toxicity Testing, Stem Cell Biology

Restrictions for research use only. Intended for use by laboratory professionals.

Methylcellulose-based medium

Sterile-filtered

Stable until expiry date on label. Do not use past expiration date.

Store at -20°C

Methylcellulose in Iscove's MDM
Fetal bovine serum
Bovine serum albumin
2-Mercaptoethanol
Recombinant human stem cell factor (SCF)
Recombinant human interleukin 3 (IL-3)
Recombinant human erythropoietin (EPO)
Recombinant human granulocyte colony-stimulating factor (G-CSF)
Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
Supplements

Liquid

Phenol Red