Description
This kit has been developed to isolate highly purified mouse Naïve/resting B cells from mouse splenocyte samples. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
No magnet, no column, no harsh chemicals
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and deplete.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
The non-B cells are labeled with optimized cocktail of biotinylated antibodies targeting CD3, CD4, CD8a, CD11b, CD11c, CD49b, Gr-1 and TER-119.
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Datasheet