PharmaOnco™ CellSort Mouse Hematopoietic Lineage Selection Kit II

This kit has been developed to isolate hf hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow by immunomagnetic positive selection. Lineage committed cells (lineage positive) include T cells, B cells, monocytes/macrophages, granulocytes, and erythrocytes are depleted by labeling biotin conjugated antibody cocktail followed by incubation with magnetic nanoparticles. Isolated cells highly expressed CD117 or Sca-1 ​are immediately available for downstream applications such as flow cytometry, counting, or other applications.

Cell Isolation

Unwanted cells are removed by immunomagnetic depletion.

This kit is available for processing 1 x 10^9 cells.

Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting s CD5, CD45R (B220), CD11b, Anti-Gr-1(Ly-6G/C), 7-4 and Ter-119.

Lineage positive Cell

Depletion

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

Mouse bone marrow

1 kit

PharmaOnco™ Biotinylated Antibody Cocktail, 1 mL
PharmaOnco™ Streptavidin beads, 2 mL

Magnetic separator

Lineage-negative cells

Mouse

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.