PharmaOnco™ CellSort Two-Step Mouse CD5+ B-1a Cell Selection Kit

B-1a cells are long-lived, self-renewing innate-like B cells that predominantly inhabit the peritoneal and pleural cavities. This kit has been developed to isolate CD5+ B-1a Cell from mouse body cavities or spleen by two-step procedure. The non-B-1a cells are deleted first and then the B-1a cells are Isolated cells ​are isolated by positive selection. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.

Cell Isolation

First step (negative selection); Next step (positive selection).

This kit is available for processing 2 x 10^9 cells.

Step 1: Non-B-1 cells; Step 2: B-1a cells.

Negative selection; positive selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

Mouse body cavities or spleen

1 kit, 20 tests

B Cells

Mouse

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.