PharmaOnco™ CHO-Tg(Human FUT4//Human CD15) Stable Cell Line, Single Clone

Creative Biolabs offers CHO-Tg(Human FUT4//Human CD15) stable cell line which the FUT4-CD15 gene stably expressed in CHO cells.

CHO-Tg(Human FUT4//Human CD15) stable cell line is a genetically modified Chinese hamster ovary (CHO) cell line that stably expresses human FUT4 gene encoding the enzyme fucosyltransferase 4 (FUT4) and human CD15 antigen. FUT4 is responsible for the synthesis of the CD15 antigen, a fucosylated carbohydrate structure found on the surface of certain cells, including leukocytes. The stable expression of FUT4 and CD15 in CHO cells allows for the production of glycoproteins or glycolipids with the CD15 antigen, which can be used for various applications, such as studying the role of CD15 in cell adhesion, migration, and signaling, or for the development of diagnostic tools or therapeutic agents targeting CD15-positive cells.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

FUT4//CD15

Human

CHO

The FUT4 gene encodes for the enzyme alpha-(1,3)-fucosyltransferase 4, which is involved in the process of adding fucose sugar molecules to glycoproteins and glycolipids. Fucosyltransferases play a role in various biological processes such as cell adhesion, migration, and signaling.
The CD15 antigen, also known as Lewis X antigen, is a carbohydrate structure that is synthesized by the FUT4 enzyme. It is a cell surface glycolipid and is commonly used as a marker for certain types of immune cells, such as neutrophils and monocytes.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.