PharmaOnco™ HEK293T-Tg(Human Gαqi5//Human Y5 Receptor) Division-Arrested Cell, Single Clone

Creative Biolabs offers HEK293T-Tg(Human Gαqi5//Human Y5 Receptor) Division-Arrested Cell which Y5 receptor stably expressed in HEK293T cells which overexpress Gαqi5.

HEK293T-Tg(Human Gαqi5//Human Y5 Receptor) Division-Arrested Cell was engineered to express the receptor human Y5 (NM_006174) and Gαqi5 gene. This cell line can be used to study Y5 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

Y5 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

Y5

Neuropeptide Y

Human

HEK293T Gαqi5

Expression vector containing full-length human NPY5R cDNA (GenBank Accession Number NM_006174) with FLAG tag sequence at N-terminus

NM_006174

The human NPY5 receptor mRNA expresses largely in the central nervous system, and highly expressed in hypothalamic and thalamic nuclei. Neuropeptide Y has a major role in the physiological control of energy homeostasis, and the NPY5 receptor is a prime candidate to mediate some of the effects through metabolic changes such as decreased lipolysis and thermogenesis, as well as hyperphagia. NPY5 is involved in both spontaneous as well as NPY-stimulated food intake and in NPY-induced reduction of blood glucose concentrations. NPY5 also plays an important role in neuroendocrine functions. It mediates the inhibitory effects of NPY on the HPT axis, and may function as part of an endogenous stress- sensing system to mediate social anxiety and motivational deficits. In addition, NPY5 may be involved in NPY-induced ischemic angiogenesis and opioid dependence and withdrawal.

2 x 10e6 cells/vial, Frozen

DMEM, 10% FBS

Cell freezing medium

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.