PharmaOnco™ HEK293-Tg(Human CD137)-NF-κB Reporter Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human CD137)-NF-κB reporter cell line which the CD137 gene stably expressed in NF-κB reporter HEK293 cells.

The HEK293-Tg(Human CD137)-NF-κB reporter cell line is a cell line that has been genetically engineered to express the human CD137 receptor and a reporter gene that is under the control of the NF-κB signaling pathway. CD137, also known as 4-1BB, is a co-stimulatory receptor that plays a key role in regulating immune responses. The NF-κB pathway is a critical signaling pathway involved in the regulation of immune and inflammatory responses. This cell line can be used to investigate the effects of various compounds or treatments on NF-κB signaling and to screen for potential therapeutic agents that modulate this pathway.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

CD137

Human

HEK293

GenBank accession #NM_001561

CD137, also known as 4-1BB, is a protein-coding gene that encodes a cell surface receptor that is part of the tumor necrosis factor receptor superfamily. CD137 is primarily expressed on activated T cells, natural killer cells, dendritic cells, and monocytes, and plays a key role in regulating immune responses. Activation of CD137 by its ligand, CD137L, leads to the activation of downstream signaling pathways that stimulate T cell proliferation, survival, and cytokine production. This activation enhances immune responses against pathogens and cancer cells. CD137 has been implicated in the modulation of autoimmune diseases, as well as in the promotion of anti-tumor immunity.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.