PharmaOnco™ HEK293-Tg(Human SIRP-α) Stable Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human SIRP-α) stable cell line which the SIRP-α gene stably expressed in HEK293 cells.

The HEK293-Tg(Human SIRP-α) stable cell line is a genetically modified cell line derived from HEK293 cells that expresses the human SIRP-α protein. SIRP-α, or signal regulatory protein alpha, is a cell surface protein that plays a critical role in regulating immune responses, particularly in the context of immune cell activation and inhibition. The stable expression of human SIRP-α in the HEK293 cell line allows for the study of the protein's function and signaling pathways in a controlled laboratory setting. This cell line can be used in various research applications, such as studying immune cell interactions, investigating immune checkpoint pathways, and developing novel therapeutics targeting the SIRP-α protein.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

SIRP-α

Human

HEK293

GenBank Accession #NM_080792

The SIRP-alpha (signal regulatory protein alpha) gene encodes a cell surface protein that plays a role in regulating immune responses. It is primarily expressed on myeloid cells and functions as a negative regulator of phagocytosis and inflammation. SIRP-alpha binds to CD47, a widely expressed cell surface protein, which sends a "do not eat me" signal to phagocytic cells. This interaction helps prevent the unnecessary phagocytosis of healthy cells and tissues. Abnormalities in the SIRP-alpha gene have been associated with various diseases, including cancer and autoimmune disorders. Research is ongoing to better understand the role of SIRP-alpha in immune regulation and develop potential therapeutic interventions targeting this pathway.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.