PharmaOnco™ Jurkat-TCR KO//B2M KO Stable Cell Line, Single Clone

Creative Biolabs offers Jurkat-TCR KO//B2M KO stable cell line which the TCR and B2M gene were knocked out in Jurkat cells.

In this Jurkat-TCR KO//B2M KO stable cell line, both the T cell receptor (TCR) and beta-2 microglobulin (B2M) genes have been knocked out using gene editing techniques. The TCR is the receptor on T cells that recognizes antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. By knocking out the TCR gene, the cells are unable to respond to antigen stimulation through this pathway. B2M is a protein that is required for the proper expression of MHC class I molecules on the cell surface. Knocking out B2M disrupts the MHC class I pathway, preventing the cells from presenting antigen to cytotoxic T cells. This double knockout stable cell line can be used in research to study T cell signaling pathways, immune responses, and the interactions between T cells and antigen-presenting cells.

Loss of gene function;
Stability and Specificity;
Reliability and reproducibility;

Functional assay

TCR//B2M

Human

Jurkat

The TCR gene, or T-cell receptor gene, is a gene responsible for encoding the protein receptors on the surface of T cells. T cells are a type of white blood cell that play a central role in the immune system, recognizing and responding to foreign antigens such as viruses, bacteria, and cancer cells. There are two main types of TCR genes, alpha and beta, which combine to form the T-cell receptor complex. These receptors are highly diverse and can recognize a wide variety of antigens, allowing T cells to mount specific immune responses to different threats. Mutations or dysregulation of the TCR gene can lead to immune system dysfunction, resulting in conditions such as autoimmune diseases, immunodeficiency disorders, and cancer. Studying the TCR gene and its role in immune function is important for understanding and developing treatments for these conditions.
The B2M gene encodes the Beta-2 microglobulin protein, which plays a role in the immune system by forming part of the major histocompatibility complex (MHC) class I molecule. This molecule is important for presenting antigens to T cells, allowing the immune system to recognize and destroy infected or abnormal cells. Mutations in the B2M gene can lead to various immune system disorders, including autoimmune diseases and immunodeficiency. Additionally, elevated levels of Beta-2 microglobulin in the blood have been associated with certain types of cancer, such as multiple myeloma and lymphoma.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.