Lipid Kinase Panel Screening Service
Background Service Highlights FAQs Contact Us
Introduction to Lipid Kinases and Their Pivotal Role in Biological Systems
Lipid kinases are a specialized class of enzymes critical for various cellular functions, operating at the nexus of signal transduction, membrane dynamics, and protein regulation. These enzymes catalyze the precise phosphorylation of lipid molecules, generating a diverse array of phosphorylated lipid second messengers. These lipid messengers, in turn, orchestrate a wide spectrum of cellular processes by selectively binding to and modulating the activity of numerous effector proteins. This intricate regulatory network underscores their fundamental importance in maintaining cellular homeostasis and responding to external stimuli.
Lipid Kinase Panel Screening Service at Creative Biolabs
At Creative Biolabs, we leverage over two decades of expertise in biological research to offer state-of-the-art Lipid Kinase Panel Screening Services. Our service is meticulously designed to support drug discovery and target identification initiatives by providing comprehensive insights into compound interactions with a broad spectrum of lipid kinase enzymes. Understanding how novel compounds modulate these critical enzymes is a key step in developing new therapies.
Our commitment at Creative Biolabs is to deliver high-quality, reproducible data efficiently, enabling researchers to make informed decisions swiftly. We pride ourselves on offering a robust and continuously expanding panel of lipid kinase targets, ensuring our clients have access to the most relevant and scientifically validated assays for their research endeavors.
Service Content and Workflow
Creative Biolabs' Lipid Kinase Panel Screening Service is a multifaceted offering built upon advanced assay technologies and a streamlined workflow, ensuring precision and reliability.
Comprehensive Kinase Panel and Assay Formats
Our service features a diverse panel of lipid kinases, including critical members such as various PI3Ks and other related kinases. We offer customizable kinase panels, allowing researchers to select targets most relevant to their specific therapeutic areas or biological processes.
Our primary biochemical kinase screening employs gold-standard radioactive assay formats. These methods directly measure enzyme activity by quantifying ADP production or phosphate incorporation, delivering highly accurate data and minimizing the incidence of false positives or negatives that can plague less direct assay methodologies. This ensures that every result provided by Creative Biolabs is a true reflection of the compound's impact on kinase activity.
Flexible Screening Conditions and High Throughput Capabilities
At Creative Biolabs, we understand that optimal screening conditions vary depending on the kinase and compound characteristics. Therefore, our services allow for flexibility in ATP concentrations, including physiologically relevant concentrations (e.g., 1mM), to accurately mimic in vivo conditions. We can screen compounds against various inhibitor types, including ATP-competitive, substrate competitive, and allosteric modulators, providing a holistic view of compound mechanism of action.
Our facilities are equipped for low, medium, and high-throughput screening, accommodating projects of varying scales. We offer comprehensive services including cherry-picking, efficient compound management, and subsequent IC50 (inhibitor concentration at 50% inhibition) follow-up studies, ensuring a seamless transition from initial hits to validated lead compounds.
Cellular Kinase Activity and Target Engagement
Beyond biochemical assays, Creative Biolabs provides advanced cellular kinase activity screening using phosphorylation assays. Furthermore, we offer cellular kinase target engagement screening through cutting-edge technologies like Cellular Thermal Shift Assays. These cellular approaches provide crucial insights into how compounds behave within a more physiological context, confirming in vitro findings and assessing cellular permeability and target binding.
Downstream Analysis and Custom Assay Development
Our services extend to signaling pathway analysis and phospho-proteomics, allowing for the assessment of inhibitor modulation and the identification of potential biomarkers. Creative Biolabs' expertise also includes the development of custom kinase assays for targets not currently in our extensive portfolio, encompassing every step from plasmid construction and protein expression to purification and assay optimization. This bespoke service ensures that even the most unique research needs are met with scientific rigor.
Our Advantages
Creative Biolabs stands out as a premier partner in kinase research due to several distinct advantages that underscore our commitment to scientific excellence and client satisfaction.
-
Deep Expertise
-
Customization and Flexibility
-
Cost-Effectiveness and Quality
FAQs
Q1: What types of compounds can be screened?
A1: Creative Biolabs can screen a wide range of compounds, including small molecules, natural products, and peptides, provided they meet our specified solubility and concentration requirements.
Q2: What information is required for compound submission?
A2: Clients are typically required to provide details on compound concentration, solvent, molecular weight, and any specific handling instructions. Detailed submission guidelines for both academic and for-profit customers are available upon request, including shipping information to our facilities.
Q3: Can Creative Biolabs develop assays for novel or unlisted lipid kinases?
A3: Yes, Creative Biolabs specializes in custom assay development. We can develop novel assays for lipid kinases not currently in our panel, a service that includes gene cloning, protein expression and purification, and full assay optimization.
Contact Us
To learn more about Creative Biolabs' advanced Lipid Kinase Panel Screening Services or to discuss your specific research needs, please do not hesitate to contact our expert team. We are committed to partnering with you to accelerate your drug discovery initiatives.
For Research Use Only | Not For Clinical Use
