MetaboStat™ BCA Protein Concentration Assay Kit

CAT#: ITS-0326-YL45
Product Type: Assay Kit
Species: All Species
Brand: MetaboStat™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies protein concentration using a colorimetric BCA method.
Brand
MetaboStat™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
Utilizing the highly sensitive bicinchoninic acid methodology, this colorimetric assay kit provides rapid and precise quantitative determination of total protein concentrations across diverse biological samples. The optimized formulation features an expansive linear detection range and excellent compatibility with common laboratory detergents. Adaptable for both high-throughput microplate and standard cuvette formats, it delivers exceptional stability and reproducibility, establishing a reliable foundation for downstream molecular and biochemical investigations.
Features
Delivers a highly sensitive, detergent-compatible colorimetric formulation with a broad linear range, enabling rapid and precise high-throughput protein quantification across diverse biological samples.
Process Relevance
Quantifying total protein concentration to ensure consistency and yield optimization during biomolecule extraction and purification.
Application Stage
Basic research, downstream processing, and analytical testing
Applications
Enables accurate colorimetric quantification of total protein in cell lysates and tissue homogenates prior to downstream assays.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Mammalian samples, bacteria, tissues, cell lysates, purified proteins
Research Areas
Biochemistry; Molecular Biology; Proteomics; Cell Biology
Data Output Type
Absorbance (OD)
Species
All Species
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on dry ice under temperature-controlled conditions.
Background
Accurate determination of protein concentration is a critical prerequisite for quantitative molecular biology techniques. The bicinchoninic acid methodology relies on the reduction of copper(II) to copper(I) by peptide bonds under alkaline conditions, followed by the highly sensitive, stoichiometric chelation of BCA with the reduced copper to yield a stable chromophore.
For Research Use Only | Not For Clinical Use
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