CytoFinal™ Standard Human Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB87
Product Type: ELISA Kit
Species: Human
Target: Human cytoplasmic Aspartate Aminotransferase (AST, GOT1) protein
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in human serum, plasma, tissues, and cell lysates.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human AST (GOT1) ELISA Kit enables highly sensitive and specific in vitro quantification of cytoplasmic Aspartate Aminotransferase in human biological samples. It supports reliable detection in serum, plasma, tissue homogenates, cell lysates, and culture supernatants, facilitating research across hepatology, oncology, and immunology.
Features
High-sensitivity detection of human cytoplasmic AST (GOT1) with a lower limit of 5.5 ng/mL for accurate measurements.
Excellent specificity ensures minimal cross-reactivity or interference from analogues, enhancing assay reliability across sample types.
Lyophilized standard format provides stability up to 12 months, supporting consistent results with proper storage and handling.
Sandwich ELISA format with colorimetric detection allows straightforward quantification and compatibility with standard laboratory equipment.
Process Relevance
Monitors cytoplasmic AST levels to assess liver function, cellular metabolism, and immune response in research studies.
Application Stage
Applicable in drug discovery, biomarker validation, and preclinical or clinical sample evaluation.
Applications
Suitable for ELISA-based assays in serum, plasma, tissue, cell lysates, or supernatants to quantify GOT1 and support downstream ADME profiling studies.
Target
Human cytoplasmic Aspartate Aminotransferase (AST, GOT1) protein
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzymology, hepatology, tumor immunology, infection immunity, pharmacokinetics (ADME), cancer research, metabolic pathway studies, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA.
Assay Range
15.6 ng/ml - 1000 ng/ml
LOD
< 5.50 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aspartate Aminotransferase (GOT1) catalyzes reversible transamination between aspartate and α-ketoglutarate, essential for amino acid metabolism. GOT1 levels reflect hepatocellular integrity and metabolic activity, serving as biomarkers for liver and disease research.
Pathways
GOT1 participates in amino acid and nitrogen metabolism, linking the TCA cycle with gluconeogenesis. Altered activity can influence energy balance, redox status, and cellular signaling pathways.
For Research Use Only | Not For Clinical Use
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