MetaVital™ Rat Dimethylaniline Monooxygenase [N-Oxide-Forming] 4 (FMO4) ELISA Kit

CAT#: ITS-0326-WB20
Product Type: ELISA Kit
Species: Rat
Target: Rat Dimethylaniline Monooxygenase [N-Oxide-Forming] 4 (FMO4)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures Rat FMO4 levels in tissue homogenates, cell lysates, and biological fluids.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Rat FMO4 ELISA Kit provides a sensitive in vitro solution for precise quantification of FMO4 concentrations. It enables accurate assessment in tissue homogenates, cell lysates, and other biological samples, supporting enzymatic research and metabolic studies.
Features
High specificity detection of native Rat FMO4 protein, minimizing cross-reactivity with related flavin-containing monooxygenases.
Colorimetric readout allows quantitative measurements ranging from 0.156 ng/mL to 10 ng/mL with consistent precision.
Lyophilized standards ensure long-term stability and reproducibility, facilitating reliable comparison across experiments.
Optimized for multiple sample types including tissue homogenates, cell lysates, and biological fluids.
Process Relevance
Supports accurate monitoring of FMO4 activity for metabolic enzyme research and drug metabolism studies.
Application Stage
Drug discovery, pharmacokinetics analysis, and preclinical metabolic research.
Applications
Used for quantitative ELISA measurements in rat tissues, cellular lysates, and biological fluids; facilitates enzyme activity profiling, ADME profiling studies, and metabolic regulation studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Rat Dimethylaniline Monooxygenase [N-Oxide-Forming] 4 (FMO4)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism, pharmacokinetics (ADME), xenobiotic biotransformation, enzymology, toxicology, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement via colorimetric endpoint assay.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Keep containers tightly sealed and avoid prolonged exposure to direct sunlight or excessive heat. Ensure components are stored in a dry, well-ventilated area to maintain stability throughout the shelf life.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Flavin-containing monooxygenases (FMO) are crucial for oxidative metabolism of xenobiotics, converting nitrogen- and sulfur-containing compounds to N- or S-oxides. FMO4 specifically contributes to hepatic biotransformation and detoxification in rats, impacting pharmacokinetics and toxicology studies.
Pathways
FMO4 participates in oxidative xenobiotic metabolism, acting downstream of flavin adenine dinucleotide (FAD)-dependent electron transfer. It interacts with NADPH as a cofactor and contributes to N- and S-oxidation pathways, regulating drug biotransformation and detoxification processes.
For Research Use Only | Not For Clinical Use
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