MetaVital™ Rat Glutathione S Transferase Alpha 5 (GSTA5) ELISA Kit

CAT#: ITS-0326-WB36
Product Type: ELISA Kit
Species: Rat
Target: Rat Glutathione S-transferase alpha-5 (GSTA5)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures GSTA5 levels in rat tissues, cell lysates, and biological fluids using ELISA methodology.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Rat GSTA5 ELISA Kit provides a robust in vitro platform for precise quantification of Glutathione S-transferase alpha-5 in rat tissue homogenates, cell lysates, and biological fluids, enabling high-sensitivity enzymatic research and translational studies.
Features
High specificity for native rat GSTA5 ensures reliable detection in complex biological matrices without cross-reactivity.
Colorimetric detection enables straightforward quantification across a broad dynamic range of 0.156-10 ng/mL.
Kit stability optimized under recommended storage, maintaining <5% activity loss until expiration for consistent experimental results.
Comprehensive assay components for 96 tests, facilitating reproducible, standardized measurements across multiple samples.
Process Relevance
Enables accurate monitoring of GSTA5 activity to support enzymology studies and hepatotoxicity assessments in preclinical research.
Application Stage
Drug discovery, enzymatic profiling, and in-process preclinical study validation.
Applications
Designed for quantitative ELISA measurement of GSTA5 in tissue homogenates, cell lysates, and various biological fluids for ADME profiling studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Rat Glutathione S-transferase alpha-5 (GSTA5)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzymology, pharmacokinetics (ADME), hepatology, cancer research, tumor immunity, preclinical translational studies, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of enzyme concentration within biological samples.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GSTA5 is a member of the alpha-class glutathione S-transferases, critical for detoxification and cellular defense against oxidative stress. Its regulation provides insight into liver function, xenobiotic metabolism, and cancer-related enzyme expression patterns.
Gene ID
NOS operates within the L-arginine-nitric oxide pathway, utilizing NADPH and oxygen to generate NO. This downstream signal activates soluble guanylate cyclase, increasing cGMP levels to modulate vascular tone and immune responses.
Pathways
GSTA5 participates in glutathione-mediated detoxification, catalyzing conjugation of glutathione to electrophilic compounds. It interacts with oxidative stress signaling pathways and influences hepatic metabolic processes.
For Research Use Only | Not For Clinical Use
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