CytoFinal™ Chicken Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB100
Product Type: ELISA Kit
Species: Chicken
Target: Mitochondrial aspartate aminotransferase (AST/GOT2)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures mitochondrial AST (GOT2) levels in chicken tissues, cells, and biological fluids with high sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
The CytoFinal™ Chicken AST (GOT2) ELISA Kit provides a robust in vitro solution for precise quantification of mitochondrial aspartate aminotransferase in tissue homogenates, cell lysates, and biological fluids, enabling high-fidelity research into metabolic and liver-associated processes.
Features
Sandwich ELISA format ensures high specificity and reproducibility for mitochondrial AST (GOT2) detection in complex samples.
Exceptional sensitivity, detecting concentrations as low as 24 pg/mL across a dynamic range of 78-5000 pg/mL.
Lyophilized standards and reagents provide long-term stability with minimal activity loss under proper storage conditions.
Colorimetric detection allows quantitative measurement with straightforward readout using standard microplate readers.
Process Relevance
Supports accurate mitochondrial enzyme profiling to optimize metabolic studies and assess tissue-specific enzymatic activity.
Application Stage
Drug discovery, metabolic research, and in vitro tissue or cell-based experimental studies.
Applications
Applicable to quantitative ELISA analyses of chicken tissue homogenates, cultured cell lysates, serum, plasma, and other biological fluids for ADME profiling studies.
Target
Mitochondrial aspartate aminotransferase (AST/GOT2)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Mitochondrial metabolism, pharmacokinetics (ADME), hepatology, enzyme kinetics, veterinary physiology, avian metabolic research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of mitochondrial AST (GOT2) enzymatic levels in biological samples.
Assay Range
78 pg/ml - 5000 pg/ml
LOD
< 24 pg/ml
Species
Chicken
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Mitochondrial aspartate aminotransferase (GOT2) plays a central role in the malate-aspartate shuttle, critical for energy metabolism and amino acid homeostasis. Dysregulation of GOT2 is linked to metabolic disorders, hepatocyte dysfunction, and mitochondrial pathophysiology, making it a vital biomarker for biochemical and veterinary research.
Pathways
GOT2 catalyzes the reversible transfer of amino groups between glutamate and oxaloacetate, connecting the TCA cycle to amino acid metabolism. Its activity influences mitochondrial NADH/NAD+ balance and downstream ATP production, linking central carbon metabolism with nitrogen homeostasis.
For Research Use Only | Not For Clinical Use
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