MMP-9 Assay Portfolio Service
Cancer biomarkers play an important role in fields, such as cancer prognosis and diagnosis, predicting disease recurrence, monitoring disease progression, predicting and monitoring treatment efficacy, and cancer screening. Matrix Metalloproteinase-9 (MMP-9) is a potential biomarker for various cancers. With advanced and high-end technologies, rich experienced scientists, Creative Biolabs is an excellent service provider in the field of tumor marker assay. After long years ahead to fully comprehend tumor marker, we launch our MMP-9 assay portfolio service which can be useful for targeted cancer therapy and diagnosis.
Introduction What We Offer Publication Why Choose Us FAQs Customer Review Related Services Contact Us
Characteristics of MMP-9
MMP-9 is a member of matrix metalloproteinases (MMPs) which are a family of zinc-dependent endopeptidases with more than 20 different members, such as collagenases, gelatinases, matrilysins, stromelysins and membrane. The gene of human MMP-9 is located at chromosome 20q13.12, which contains 13 exons and 12 introns. Human MMP-9 protein contains the catalytic domain, hemopexin-like domain, signal peptide, propeptide region and the hinge region. The catalytic domain of MMP-9 contains active site, fibronectin type II (FN2) domains and zinc-binding region. This protein contains five calcium ions and two zinc ions.
Fig.1 Schematic illustration of domain structures and motifs in MMP-9. 1, 3
A Precision Portfolio for Unprecedented Insights
At Creative Biolabs, we understand that a single assay is rarely sufficient to capture the full picture of MMP-9 activity. Our MMP-9 assay portfolio is meticulously designed to provide a panoramic view of this protein's functions, offering unparalleled flexibility and accuracy. Our service suite includes:
Invasion & Metastasis Assays
Evaluate the ability of cancer cells to invade through a biological matrix, directly assessing the impact of MMP-9 on a primary mechanism of malignancy.
Angiogenesis Assays
Quantify the formation of new blood vessels, a key process where MMP-9 plays a pivotal role.
Migration Assays
Measure the directed movement of cells, providing insight into the role of MMP-9 in cellular motility.
Cytokine & Proliferation Assays
Characterize the influence of MMP-9 on a broader range of cellular behaviors, including cell growth and the cytokine microenvironment.
Discover how our tailored MMP-9 assay solutions can accelerate your oncology project and validate your therapeutic hypothesis. Request a consultation to learn more.
Publication
This review discusses the complex and dual role of MMP9 in cancer and its challenges as a therapeutic target. While it often promotes tumor invasion, metastasis, and angiogenesis, MMP9 can also have tumor-suppressive functions. The article examines its regulation, its wide range of substrates, and its involvement in the tumor microenvironment. It also reviews various strategies to inhibit MMP9, highlighting the difficulties and failures in clinical trials due to toxicity and lack of specificity, and concludes that more selective targeting approaches are needed.
Fig.2 Signaling pathways regulating MMP9 expression. 2, 3
Why Choose Us?
Creative Biolabs offers comprehensive services for MMP-9 in cancer research. We have deep scientific expertise in MMP-9's dual roles and the challenges of achieving selective inhibition. Our multidisciplinary approach provides insights beyond single-assay results, helping clients navigate the complexity of MMP-9's function in promoting tumor growth, metastasis, and epithelial-to-mesenchymal transition (EMT), while also acknowledging its tumor-suppressive functions.
Choosing Creative Biolabs means partnering with a team of experts who have deep scientific expertise in MMP-9 and its role in cancer. We help you navigate the complexity of MMP-9's dual roles and the challenges of achieving selective inhibition.
Experience the Creative Biolabs advantage—get a quote today and let us partner with you to unlock the full potential of your research.
Frequently Asked Questions
Q1: What is the benefit of your functional assays over simple western blotting or qPCR?
A1: While western blotting and qPCR measure protein and gene expression levels, our functional assays directly measure outcomes like cell invasion and migration. This validates whether an observed change in expression translates to a meaningful change in tumor behavior.
Q2: How do you handle assay development for non-standard MMP-9 variants or isoforms?
A2: We understand that MMP-9 can exist in various forms, including pro- and activated forms. Our team can develop custom assays to specifically analyze the activity of these different variants, ensuring that your research is precise and comprehensive.
Q3: Do you offer services to analyze the interaction of MMP-9 with its inhibitors at a molecular level?
A3: In addition to functional assays, we can employ techniques to characterize the binding kinetics and affinity of your compounds to MMP-9. This provides crucial molecular-level data to complement the functional insights from our assays.
Customer Review
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In-depth Mechanistic Understanding
We partnered with Creative Biolabs to investigate a novel therapeutic antibody. The report went beyond simple data, giving us a clear mechanistic explanation for how our antibody impacted MMP-9 activity in our in vitro models. - Sam*l
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Cross-Platform Validation
Their ability to combine MMP-9 activity assays with other readouts, like angiogenesis, was invaluable. It allowed us to validate our findings across different biological processes, strengthening our preclinical data package. - Li*o
Related Services
To further support your research, we recommend considering these complementary services:
CCR Panel Screening Service
Creative Biolabs provides comprehensive cellular functional selectivity panels, including a chemokine receptor (CCR) panel screening service, to explore the therapeutic potential of CCR targets.
Learn More →
Toxicity Assessment by Quantification of Total Protein
Creative Biolabs offers a complete portfolio of toxicity assessment assays, including the quantification of total protein. Our advanced platforms and powerful technology provide high-quality analytical development and qualification services to assist customers worldwide.
Learn More →
How to Contact Us
The Creative Biolabs MMP-9 assay portfolio is more than just a service—it's a partnership. We provide the expertise, technology, and comprehensive data you need to confidently navigate the complexities of MMP-9 research.
Inquire Now to connect with our specialists and learn how we can help you accelerate your project and achieve your scientific goals.
References
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Huang, Hao. "Matrix Metalloproteinase-9 (MMP-9) as a Cancer Biomarker and MMP-9 Biosensors: Recent Advances." Sensors 18.10 (2018). https://doi.org/10.3390/s18103249
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Augoff, Katarzyna, et al. "MMP9: A Tough Target for Targeted Therapy for Cancer." Cancers 14.7 (2022). https://doi.org/10.3390/cancers14071847
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Distributed under Open Access license CC BY 4.0, without modification.
