Short Description
Quantifies ADA activity using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Adenosine Deaminase Assay Kit provides a robust, high-throughput method for measuring enzymatic activity in diverse biological samples. Utilizing a kinetic spectrophotometric approach, the system quantifies the deamination of adenosine to inosine through a series of coupled enzymatic reactions. This reliable tool enables researchers to investigate purine metabolism and immune signaling pathways, offering high sensitivity and reproducibility for fundamental biochemical studies and metabolic screening applications.
Features
Employs a kinetic enzymatic reaction for rapid, sensitive ADA quantification, facilitating high-throughput metabolic research and enzyme inhibitor screening in various biological samples.
Process Relevance
Quantifying purine metabolism to evaluate cellular health and enzymatic pathway stability in research models.
Application Stage
Drug discovery, metabolic research, and enzyme inhibitor screening.
Applications
Quantify enzymatic activity in cell lysates and biological fluids to evaluate purine metabolism and immune signaling pathways.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants.
Research Areas
Immunology; Purine Metabolism; Metabolic Research
Datasheet