ModifiSite™ Adenosine Deaminase Assay Kit

CAT#: ITS-1125-YL1
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies ADA activity using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Adenosine Deaminase Assay Kit provides a robust, high-throughput method for measuring enzymatic activity in diverse biological samples. Utilizing a kinetic spectrophotometric approach, the system quantifies the deamination of adenosine to inosine through a series of coupled enzymatic reactions. This reliable tool enables researchers to investigate purine metabolism and immune signaling pathways, offering high sensitivity and reproducibility for fundamental biochemical studies and metabolic screening applications.
Features
Employs a kinetic enzymatic reaction for rapid, sensitive ADA quantification, facilitating high-throughput metabolic research and enzyme inhibitor screening in various biological samples.
Process Relevance
Quantifying purine metabolism to evaluate cellular health and enzymatic pathway stability in research models.
Application Stage
Drug discovery, metabolic research, and enzyme inhibitor screening.
Applications
Quantify enzymatic activity in cell lysates and biological fluids to evaluate purine metabolism and immune signaling pathways.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants.
Research Areas
Immunology; Purine Metabolism; Metabolic Research
Size
100 tests
Data Output Type
Quantitative enzyme activity measurement (endpoint)
Assay Time
50 min
LOD
LOD: 0.3 U/L
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
ADA is a cytosolic enzyme crucial for purine catabolism, catalyzing the conversion of adenosine to inosine and ammonia. Its primary function is regulating the concentration of adenosine and deoxyadenosine, which are potent signaling and cytotoxic molecules. Dysregulation of ADA activity is a key indicator and driver of immunological disorders and chronic inflammation.
Pathways
ADA is a key enzyme in the purine salvage pathway, regulating adenosine levels to prevent toxic accumulation of deoxyadenosine. It acts as an upstream modulator of adenosine receptor signaling, influencing T-cell maturation and immune homeostasis.
For Research Use Only | Not For Clinical Use
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