ModifiSite™ Glutathione Reductase Kit

CAT#: ITS-1125-HM127
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures GR activity by tracking GSSG reduction.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutathione Reductase Kit employs a DTNB reduction method to assess NADPH-dependent redox recycling in toxicology studies. This assay validates the ability of cells to regenerate reduced glutathione, a critical antioxidant. It is essential for investigating mechanisms of drug-induced oxidative stress and the maintenance of the cellular redox buffer.
Features
DTNB reduction method; Assessing NADPH-dependent redox recycling in toxicology.
Process Relevance
Redox Recycling Assessment
Application Stage
Discovery
Applications
Glutathione recycling and redox balance study.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Plasma, serum, tissue and culture media samples
Research Areas
Oxidative Stress; Toxicology
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.4 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutathione Reductase regenerates reduced glutathione (GSH) from oxidized glutathione using NADPH. It is essential for maintaining the redox homeostasis required for detoxification.
For Research Use Only | Not For Clinical Use
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