Short Description
Measures GR activity by tracking GSSG reduction.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutathione Reductase Kit employs a DTNB reduction method to assess NADPH-dependent redox recycling in toxicology studies. This assay validates the ability of cells to regenerate reduced glutathione, a critical antioxidant. It is essential for investigating mechanisms of drug-induced oxidative stress and the maintenance of the cellular redox buffer.
Features
DTNB reduction method; Assessing NADPH-dependent redox recycling in toxicology.
Process Relevance
Redox Recycling Assessment
Application Stage
Discovery
Applications
Glutathione recycling and redox balance study.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Plasma, serum, tissue and culture media samples
Research Areas
Oxidative Stress; Toxicology
Datasheet