Short Description
Quantifies hydroxyproline using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Hydroxyproline Assay Kit utilizes a specialized colorimetric technique to quantify this unique amino acid in tissue and biological fluid samples. By measuring hydroxyproline concentration after acid hydrolysis, the system provides a precise index of total collagen content. This analytical tool is indispensable for researchers investigating fibrotic processes, extracellular matrix remodeling, and connective tissue degradation, offering high reproducibility for fundamental studies in structural biology.
Features
Uses a streamlined chemical reaction for the specific quantification of hydroxyproline, enabling accurate assessment of collagen density and tissue remodeling in various preclinical research applications.
Process Relevance
Measuring hydroxyproline to assess collagen degradation and tissue remodeling in experimental research models.
Application Stage
Fibrosis research and connective tissue characterization.
Applications
Quantify hydroxyproline levels in hydrolyzed tissue samples to accurately determine collagen content and extracellular matrix remodeling during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 560nm
Sample Type
Tissue homogenates (liver, muscle, skin), cell culture media, biological fluids.
Research Areas
Fibrosis Studies; Connective Tissue Biology; Extracellular Matrix Research
Datasheet