ModifiSite™ Hydroxyproline Assay Kit

CAT#: ITS-1125-YL25
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies hydroxyproline using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Hydroxyproline Assay Kit utilizes a specialized colorimetric technique to quantify this unique amino acid in tissue and biological fluid samples. By measuring hydroxyproline concentration after acid hydrolysis, the system provides a precise index of total collagen content. This analytical tool is indispensable for researchers investigating fibrotic processes, extracellular matrix remodeling, and connective tissue degradation, offering high reproducibility for fundamental studies in structural biology.
Features
Uses a streamlined chemical reaction for the specific quantification of hydroxyproline, enabling accurate assessment of collagen density and tissue remodeling in various preclinical research applications.
Process Relevance
Measuring hydroxyproline to assess collagen degradation and tissue remodeling in experimental research models.
Application Stage
Fibrosis research and connective tissue characterization.
Applications
Quantify hydroxyproline levels in hydrolyzed tissue samples to accurately determine collagen content and extracellular matrix remodeling during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 560nm
Sample Type
Tissue homogenates (liver, muscle, skin), cell culture media, biological fluids.
Research Areas
Fibrosis Studies; Connective Tissue Biology; Extracellular Matrix Research
Size
100 Tests
Data Output Type
Quantitative concentration measurement
Assay Time
Approx. 2 hrs
LOD
LOD: 0.5 µg/mL
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This modified amino acid is almost exclusively found in collagen and serves as a reliable marker for collagen content and turnover. Its quantification is essential for studying tissue remodeling and the biochemical composition of the extracellular matrix.
Pathways
DNA is the fundamental template for the central dogma of molecular biology, serving as the upstream source for all transcription and translation. Its quantification is essential for assessing cell proliferation and genomic stability in research studies.
For Research Use Only | Not For Clinical Use
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