ModifiSite™ Pepsin Inhibitor Assay Kit

CAT#: ITS-1125-WB26
Product Type: Assay Kit
Species: All Species
Target: Quantitative detection of pepsin (EC 3.4.23.1) activity and the evaluation of potential inhibitory molecules.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies target protein phosphorylation levels using an enzyme-linked immunosorbent assay (ELISA).
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Pepsin Inhibitor Assay Kit offers a high-throughput, non-radioactive solution for screening potential pepsin inhibitors in drug discovery. Utilizing a quenched FITC-labeled substrate, this homogeneous assay measures fluorescence recovery upon enzymatic cleavage. It provides a robust, sensitive tool for identifying therapeutic candidates targeting digestive disorders and gastric reflux pathologies.
Features
Homogeneous "Mix-Measure" Protocol: Streamlined workflow eliminates tedious wash steps and reagent transfers, significantly reducing experimental variability and manual labor.
High-Throughput Compatibility: Featuring a robust Z' factor > 0.7, this assay is optimized for automated screening of thousands of compounds daily.
Dual-Readout Versatility: Specifically designed to support both standard fluorescence and fluorescence polarization (FP) for enhanced data reliability and precision.
Ultra-Sensitive FITC Chemistry: Utilizes a highly quenched synthetic substrate that provides superior signal-to-noise ratios upon specific proteolytic cleavage.
Process Relevance
This kit enables precise screening of lead compounds to optimize inhibitory potency and ensure consistency in pharmaceutical development.
Application Stage
Critical for high-throughput drug discovery, lead compound profiling, and iterative process development within preclinical research pipelines.
Applications
Ideal for screening synthetic libraries, evaluating natural extracts, and performing kinetic inhibitor profiling to identify potent candidates for gastric and metabolic disease therapies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Quantitative detection of pepsin (EC 3.4.23.1) activity and the evaluation of potential inhibitory molecules.
Detection Method
Fluorescence Detection or Fluorescence Polarization (Ex 485 nm / Em 530 nm)
Sample Type
Chemical compounds
Research Areas
Gastrointestinal pathophysiology, oncology therapeutics, metabolic disorders, digestive enzyme kinetics, etc.
Size
200 tests
Data Output Type
Quantitative measurement of enzyme inhibition through endpoint or kinetic fluorescence intensity and polarization analysis.
Assay Time
45 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Pepsin is the primary gastric aspartic protease responsible for dietary protein degradation. Dysregulation of its activity is critically linked to peptic ulcers and reflux diseases, making it a vital therapeutic target.
Pathways
Pepsin initiates the gastric proteolytic cascade, converting proteins into peptides for downstream hydrolysis by trypsin and chymotrypsin. It operates within the catabolic pathways governing systemic nutrient absorption and protein turnover.
For Research Use Only | Not For Clinical Use
Online Inquiry