Short Description
Quantifies target protein phosphorylation levels using an enzyme-linked immunosorbent assay (ELISA).
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Pepsin Inhibitor Assay Kit offers a high-throughput, non-radioactive solution for screening potential pepsin inhibitors in drug discovery. Utilizing a quenched FITC-labeled substrate, this homogeneous assay measures fluorescence recovery upon enzymatic cleavage. It provides a robust, sensitive tool for identifying therapeutic candidates targeting digestive disorders and gastric reflux pathologies.
Features
Homogeneous "Mix-Measure" Protocol: Streamlined workflow eliminates tedious wash steps and reagent transfers, significantly reducing experimental variability and manual labor.
High-Throughput Compatibility: Featuring a robust Z' factor > 0.7, this assay is optimized for automated screening of thousands of compounds daily.
Dual-Readout Versatility: Specifically designed to support both standard fluorescence and fluorescence polarization (FP) for enhanced data reliability and precision.
Ultra-Sensitive FITC Chemistry: Utilizes a highly quenched synthetic substrate that provides superior signal-to-noise ratios upon specific proteolytic cleavage.
Process Relevance
This kit enables precise screening of lead compounds to optimize inhibitory potency and ensure consistency in pharmaceutical development.
Application Stage
Critical for high-throughput drug discovery, lead compound profiling, and iterative process development within preclinical research pipelines.
Applications
Ideal for screening synthetic libraries, evaluating natural extracts, and performing kinetic inhibitor profiling to identify potent candidates for gastric and metabolic disease therapies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Quantitative detection of pepsin (EC 3.4.23.1) activity and the evaluation of potential inhibitory molecules.
Detection Method
Fluorescence Detection or Fluorescence Polarization (Ex 485 nm / Em 530 nm)
Sample Type
Chemical compounds
Research Areas
Gastrointestinal pathophysiology, oncology therapeutics, metabolic disorders, digestive enzyme kinetics, etc.
Datasheet