ModifiSite™ Total Protein Quantification Kit

CAT#: ITS-1125-YL26
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Cell-Based Assay Development
Short Description
Quantifies protein levels using a fluorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Cell-Based Assay Development
Description
This ModifiSite™ Total Protein Quantification Kit offers a sensitive colorimetric method for the rapid quantification of total protein concentration in biological samples. Utilizing an improved Coomassie Blue reagent, the system provides a stable signal and minimizes interference from common laboratory detergents and buffers. This versatile tool is essential for researchers performing routine protein quantification, enzyme normalization, and sample characterization, ensuring high accuracy and high-throughput compatibility.
Features
Provides a robust, detergent-resistant protein quantification system that ensures high accuracy in complex lysis buffers, supporting consistent normalization for Western blotting and enzyme assays.
Process Relevance
Determining total protein concentration to ensure accurate normalization of metabolic and enzymatic data.
Application Stage
Protein purification monitoring and general laboratory quantification.
Applications
Determine total protein concentration in biological samples for normalization of assay data and monitoring of protein purification processes.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 595nm (Bradford)
Sample Type
Cell lysates, serum, plasma, biological fluids.
Research Areas
Proteomics; Molecular Biology; Biochemistry
Size
500 tests
Data Output Type
Quantitative concentration measurement
Assay Time
2 min
LOD
LOD: 60 µg/mL
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Accurate protein quantification is a fundamental requirement in almost all biochemical and molecular biology workflows. Choosing a method that is resistant to common laboratory detergents and reducing agents ensures reliable results across diverse sample types.
Pathways
Proteins are the functional effectors of almost all signaling pathways and metabolic processes. Total protein concentration reflects the downstream balance between protein synthesis (translation) and degradation (proteolysis) within a biological system.
For Research Use Only | Not For Clinical Use
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