Short Description
Quantifies protein levels using a fluorimetric method.
Development Stage
Research-Grade Cell-Based Assay Development
Description
This ModifiSite™ Total Protein Quantification Kit offers a sensitive colorimetric method for the rapid quantification of total protein concentration in biological samples. Utilizing an improved Coomassie Blue reagent, the system provides a stable signal and minimizes interference from common laboratory detergents and buffers. This versatile tool is essential for researchers performing routine protein quantification, enzyme normalization, and sample characterization, ensuring high accuracy and high-throughput compatibility.
Features
Provides a robust, detergent-resistant protein quantification system that ensures high accuracy in complex lysis buffers, supporting consistent normalization for Western blotting and enzyme assays.
Process Relevance
Determining total protein concentration to ensure accurate normalization of metabolic and enzymatic data.
Application Stage
Protein purification monitoring and general laboratory quantification.
Applications
Determine total protein concentration in biological samples for normalization of assay data and monitoring of protein purification processes.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 595nm (Bradford)
Sample Type
Cell lysates, serum, plasma, biological fluids.
Research Areas
Proteomics; Molecular Biology; Biochemistry
Datasheet