Mouse ICAM1 ELISA Kit, Lot 21AO-882 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse ICAM1 in Serum, Plasma, Cell Culture Supernatant.

This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

ELISA

ICAM-1

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Serum, Plasma, Cell Culture Supernatant

SICAM-1 / CD54, Mouse

Soluble

Plate, Standard, Diluent

Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water. Polypropylene test tubes for dilution.

1.27 pg/mL

100 μL

3 - 4 h

Pre-coated

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

4 °C

Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C). Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling. Unopened Kit: Store at 2 - 8 °C (See expiration date on the label). Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C. Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles. Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C). Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling. Unopened Kit: Store at 2 - 8 °C (See expiration date on the label). Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C. Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles. Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

For Research Use only

ICAM-1/CD54

BB2; CD54; P3.58; Icam-1; Ly-47; MALA-2; ICAM; ICAM-1; ICAM1; intercellular adhesion molecule 1; intercellular adhesion molecule-1; ICAM1; Icam1; ICAM-1

Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein which is typically expressed on endothelial cells and cells of the immune system and plays a key role in leukocyte migration and activation. It binds to integrins of type CD11a/CD18, or CD11b/CD18 and is also exploited by rhinovirus as a receptor. Soluble ICAM-1 (sICAM-1) promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage.

15894

P13597

Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling