PharmaOnco™ Human Th2 Cell Differentiation Kit

CAT#: ITS-0622-YT367
Product Type: Cell Isolation Kit
Species: Human
Description
PharmaOnco™ Human Th2 Cell Differentiation Kit is a set of optimized reagents needed to induce Th2 differentiation. It is designed to generate a highly enriched population of Th2 polarized T cells.
Applications
Cell Culture, Differentiation, Expansion
Application Notes
This kit provides sufficient reagents for the differentiation of one 24-well plate or 125 tests in a 96-well format.
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Size
1 kit
Components
Mouse Anti-Human CD3 Antibody
Human Th2 Differentiation Supplements
Reconstitution Buffers
Wash Buffer (20X)
Assay Procedure
Coat a plate with Mouse Anti-Human CD3 Antibody
Isolate human naïve CD4+ T cells from a PBMC preparation
Culture naïve CD4+ T cells in Human Th2 Differentiation Media for 13 days
Refresh the Human Th2 Differentiation Media every 3-4 days
Analyze culture media and cells for a Th2-specific cytokine production profile
Cell Background
T helper type 2 (Th2) cells
Species
Human
Format
Liquid
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store the unopened product at 2 - 8 °C.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
For Research Use Only | Not For Clinical Use
Online Inquiry