OncoIHC™ APOL1 Ready-To-Use IHC Kit

This IHC kit offers a complete solution for APOL1 staining, providing all necessary reagents in a ready-to-use format, along with a simplified protocol and optimized reagents for efficient and reliable results.

Human apolipoprotein L1 (APOL1) is a minor component of plasma high-density lipoprotein (HDL) particles and interacts with apolipoprotein A1 (ApoA1). The ApoL protein family is primarily involved in lipid transport and metabolism. APOL1 plays a role in innate immunity against Trypanosoma parasites by lysing the parasite upon activation, thereby protecting humans from infection. Genetic variants of APOL1, common in individuals of African ancestry, are linked to chronic kidney diseases such as focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 shares structural and functional similarities with Bcl-2 family proteins and may be involved in apoptosis and autophagy. Notably, APOL1 is found only in humans and a few other primates; it is absent in mice.

Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

IHC

APOL1

Human

Immunohistochemistry

FFPE tissue

Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 66
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix

To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.

APOL; APOL I; APOL1; APOL1-Specific; Apolipoprotein L; Apolipoprotein L I; Apolipoprotein L1

Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.

Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.

Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)