OncoIHC™ DNMT3A Ready-To-Use IHC Kit

For precise DNMT3A staining, utilize this ready-to-deploy IHC solution, simplifying procedures from antigen retrieval through coverslipping.

DNA methylation in vertebrates is a key epigenetic modification crucial for embryonic development, imprinting, and X chromosome inactivation. This process is catalyzed by DNA methyltransferases (DNMTs), including DNMT1, DNMT3a, and DNMT3b. Overexpression of these enzymes has been linked to cancers like gastric, urothelial, and lung cancers, potentially driving tumorigenesis and affecting patient survival. Two isoforms of DNMT3a exist: the full-length DNMT3a and a shorter variant, DNMT3a2, which is highly expressed in stem cells and tissues undergoing de novo methylation, like the testis and ovary.

Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

IHC

DNMT3A

Human, Mouse, Rat

Immunohistochemistry

FFPE tissue

Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 199
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix

To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.

DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNMT3A; DNMT3A2; M.HsaIIIA

Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.

Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.

Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)