Short Description
Improved sensitivity method for ammonia detection.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Ammonia Assay Kit II employs an NADPH-coupled enzymatic reaction, providing high sensitivity for monitoring glutaminolysis in cancer cell metabolism. This assay is critical for bioprocess monitoring, measuring ammonia accumulation that can inhibit cell growth in bioreactors. It supports the optimization of cell culture conditions and the study of nitrogen metabolism in tumor biology.
Features
NADPH-coupled enzymatic reaction; High sensitivity for monitoring glutaminolysis in cancer cell metabolism.
Process Relevance
Bioprocess Optimization
Application Stage
Upstream Processing
Applications
Bioprocess monitoring in cell culture supernatants.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Serum, Plasma, urine, and other biological samples
Research Areas
Bioprocessing; Hepatology
Datasheet