OXPHOSight™ Ammonia Assay Kit II

CAT#: ITS-1125-HM100
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Improved sensitivity method for ammonia detection.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Ammonia Assay Kit II employs an NADPH-coupled enzymatic reaction, providing high sensitivity for monitoring glutaminolysis in cancer cell metabolism. This assay is critical for bioprocess monitoring, measuring ammonia accumulation that can inhibit cell growth in bioreactors. It supports the optimization of cell culture conditions and the study of nitrogen metabolism in tumor biology.
Features
NADPH-coupled enzymatic reaction; High sensitivity for monitoring glutaminolysis in cancer cell metabolism.
Process Relevance
Bioprocess Optimization
Application Stage
Upstream Processing
Applications
Bioprocess monitoring in cell culture supernatants.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Serum, Plasma, urine, and other biological samples
Research Areas
Bioprocessing; Hepatology
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 20 min
LOD
5.3 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This kit is optimized for detecting trace levels of ammonia. It is particularly useful in cell culture where even low ammonia concentrations can alter protein glycosylation and reduce product quality.
For Research Use Only | Not For Clinical Use
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